[Histonet] products suggestions for snap freezing

Lori Richey lrichey <@t> u.washington.edu
Mon Aug 28 17:19:44 CDT 2006

I've only seen snap freezing done using liquid nitrogen.  The isopentane 
can be stored in a nalgene bottle, and a nalgene cup can be used.  Pour 
the liquid nitrogen into  a wide mouth  plastic cup, and lower slowly 
into the liquid Nitrogen.  When white plaques start forming on the 
bottom of the cup, it is cold enough to snap freeze the tissue in a cryo 

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