[Histonet] lectins

Dolores_Fischer <@t> baxter.com Dolores_Fischer <@t> baxter.com
Fri Aug 25 12:25:37 CDT 2006


Hello everyone in histoland!

I finally need to utilze the expertise of all of you fellow histonetters. 
I have limited experience in immunohistochemistry (give me a protocol and 
show me the reagents and I can follow).    I am now in a position where I 
will have to work out protocols on my own (oh no!  I will have to think!)  
Here is the first assignment.    I need to work up a protocol using 
isolectin B4 as a marker for endothelial cells in rat tissue.  Would one 
follow the application of the isolectin with an ABC reagent or, as someone 
suggested to me, use a streptavidin derivitive?  Does one need to begin 
with an antigen retreivel?  I was told that working with lectins can be 
tricky and are considered by some to be "old school" methods.   Are there 
better, newer  markers or methods for endothelial cells in rat tissue? I 
also have a reference paper that states that 100% methanol was used as a 
fixative.  Why use 100% methanol?   Is 24 hr fixation in NBF acceptable 
(or preferred)?   Any tips, comments, opinions, protocol sharing to lead 
me down the successful lectin path would be appreciated. 

Thanks and
Happy Friday to all, not much venting 
today............................well .............. those people who walk 
in from other labs and use your "stuff" and reagents 'cause they ain't got 
any can be kinda irritating...........................time for a training 
lesson...........................stay out of my kitchen and return my 
"stuff"! 

Dolores Fischer

847-270-5509 

The information transmitted is intended only for the person(s)or 
entity to which it is addressed and may contain confidential 
and/or legally privileged material. Delivery of this message to 
any person other than the intended recipient(s) is not intended in 
any way to waive privilege or confidentiality. Any review, 
retransmission, dissemination or other use of, or taking of any 
action in reliance upon, this information by entities other than 
the intended recipient is prohibited. If you receive this in 
error, please contact the sender and delete the material from any 
computer.

For Translation:

http://www.baxter.com/email_disclaimer


More information about the Histonet mailing list