[Histonet] IHC - sections falling off!

Dawson, Glen GDawson <@t> dynacaremilwaukee.com
Thu Aug 24 08:14:10 CDT 2006


Don't underestimate the power of a slide with extra adhesive properties.  Try out Goldfrost Plus Slides or the Silanized slides from Dako.

Also, try extending the time that you dry your slides before you start your procedure. 

Keep in mind that enzyme digestion can make tissue sections fall off as well.

Good Luck,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Ahmed, T
Sent: Thursday, August 24, 2006 5:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC - sections falling off!

Dear All,
I am trying to save sections of canine mammary tissue from falling off
in antigen retrieval solution in a microwave technique (have also tried
half power microwaving). I am using normal superfrost slides and leaving
them to incubate overnight.  The sections are fine after dewaxing -
therefore I believe the type of slide used whether it be superfrost or
positive charged polysaline slides should not matter? My other option is
maybe to use non heat-treatment methods of enzyme digestion such as
trypsin. Is there anyone who has any ideas to prevent sections falling
off during the microwave retrieval step? 
Any suggestions to improve are welcome. 
Thanks in advance,

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