[Histonet] IHC - sections falling off!

Ahmed, T (Tanni) Tanni.Ahmed <@t> intervet.com
Thu Aug 24 06:15:55 CDT 2006

Dear All,
I am trying to save sections of canine mammary tissue from falling off
in antigen retrieval solution in a microwave technique (have also tried
half power microwaving). I am using normal superfrost slides and leaving
them to incubate overnight.  The sections are fine after dewaxing -
therefore I believe the type of slide used whether it be superfrost or
positive charged polysaline slides should not matter? My other option is
maybe to use non heat-treatment methods of enzyme digestion such as
trypsin. Is there anyone who has any ideas to prevent sections falling
off during the microwave retrieval step? 
Any suggestions to improve are welcome. 
Thanks in advance,

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