[Histonet] double staining

cynthia haynes naje1972 <@t> yahoo.com
Tue Aug 22 09:55:21 CDT 2006


Good morning All,

I have a question for all the histochemical stainers.
I have 2 antibodies and they are Anti-Tau (3-repeat
isoform RD3)and Anti-Tau (4-repeat isoform RD4).Both
recognize Tau. Both are made in Mouse. Can I do double
staining with these antibodies? If so which should I
do first? Is it alright to use DAB and FAST
RED(VECTOR) as the chromagens? Any suggestion will be
greatly appreciated. Thank you in advance.

Cynthia Haynes H.T.



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