[Histonet] Leica DRS

[Molinari, Betsy]

No, I haven't tried that yet. But I sure will.

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)


-----Original Message-----
From: Donna Willis [mailto:donna <@t> milestonemed.com] 
Sent: Monday, August 14, 2006 11:06 AM
To: Molinari, Betsy
Subject: RE: [Histonet] Leica DRS

Betsy,
When I was the Lab Manager at Harris we had the same problem with our
Sakura
stainer.  Have you tried stirring up your paraffin before you embed.  It
seems that the polymers can separate if they set too long.  Try and see
if
this helps.  

If you are interested in a demo of the Milestone microwave processors
just
let me know.

Donna Willis,HT(ASCP)HTL
Milestone Medical
North American Application Manager
Gulf Coast Sales
2100 N. Hwy 360 Suite 506
Grand Prairie, Tx  75050
972-606-9986 office
214-725-6184 cell
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Molinari,
Betsy
Sent: Monday, August 14, 2006 10:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica DRS

Hi,

I am frustrated. I have been struggling to solve this problem and now
turn to you.

My H&E's  come out of the stainer with this blue line across them. I
have juggled times, rinses, solutions.

The slides are cut at 4-5 microns. This is my current protocol

Dryer: 20 min

Xylene x3 5 min

100 % x3 1 min

95% 1 min

rinse in tap

rinse in DH2O

 Richard Allan hematoxylin 1  1 min ( initially started w/ hematoxylin 2
but staining was too dark, I have juggled these times from 15 secs-1
min)

Rinse in tap 2 min

Richard Allan Clarifier 2  30 secs (have played w/ this time too)

Rinse in tap 2 min

Richard Allan Bluing 1 min

Rinse in tap  2 min

95 % 1 min 

Richard Allan Eosin Y 45 secs

100%- Xylenes  I min.

 The staining is acceptable..good nuclear staining but still that damn
blue streak! Any suggestions?

Thanks.

 

Betsy Molinari HT (ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave.

Houston,TX 77030

832-355-6524

832-355-6812 (fax)

 

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