[Histonet] Re: Setting Ink

RSRICHMOND <@t> aol.com RSRICHMOND <@t> aol.com
Sun Aug 13 13:08:20 CDT 2006

Karen Heckford asks >>Does anyone know if there is something that sets the 
on tissue after you have inked the margins?<< and Patsy Ruegg (along with 
several others) replies >>We use Bouin's fixative to set ink, just dip it in for 
a few seconds then go on your way.<<

Bouin's fixative contains picric acid, a potential explosion hazard that can 
give you trouble with regulatory agencies, besides being toxic, and ruinous to 
clothing (particularly since it is inclined to eat through latex).

Acetone, also often recommended, is a fire and explosion hazard.

If 2% to 3% acetic acid works - and I haven't tried it personally - why not 
use that?

If you blot the specimen dry adequately, the ink will usually stick to it - 
India ink, Davidson and similar marking inks, and tattoo inks - I've used them 
all. Ink won't stick to cauterized surfaces (such as LEEP specimens), but the 
pathologist can see these margins under the microscope whether they're inked 
or not.

Bob Richmond

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