[Histonet] paraffin wax

[Molinari, Betsy]

We do not leave our blocks in molten paraffin. If the blocks cannot be
embedded ASAP I take them out and let them cool allowing the paraffin to
harden around the tissue.
 

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anne Van
Binsbergen
Sent: Thursday, August 10, 2006 12:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] paraffin wax

another quick question
i was taught that it is not a good idea to leave tissue in molten wax
for extended periods of time - my rule of thumb has always been 4 hours
max for an 'average' size tissue and half of that for a very small
biopsy.
we have a VIP5 and a very important sample (lymph node) has been left in
the machine for over 7 hours before embedding, by a 'tardy' tech. wax
baths set to 59 celcius.
i am seriously concerned that the tissue could have been damaged by
prolonged exposure to heat. 
any comments - or am i being paranoid :-)
annie

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