[Histonet] storing frozen sections on glass slides in the freezer

[Geoff McAuliffe]

Hi Jason:

First off, I would not store, do the reaction asap after cutting the 
sections.
If you must store, I would store at -80C.  You may still get some 
reduction in the reaction, depending on what you are looking for and how
long the storage is.

Geoff

Jason Potas wrote:

> Hi everyone/anyone,
>
> I would like to store frozen rat spinal cord sections in a -20 degree C
> freezer. Does anyone have experience with the following method, ie 
> does it
> work:
>
> 1-cut sections frozen onto gelatinized glass slide.
> 2-air dry overnight at room temperature (to remove all moisture)
> 3-wrap multiple glass slides inside aluminium foil together like a 
> pack of
> chewing gum
> 4-store in the freezer at -20 deg C
> 5-when required, removed one (or required number) slide(s) from the pack,
> but without defrosting other slides
> 6-allow to reach room temperature and commence histochemistry as normal
>
> does it work without stuffing up the slides or the tissue sticking on the
> back of other slides. has someone done this?
>
> cheers
> jason
>
>


-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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