[Histonet] storing frozen sections on glass slides in the freezer

[Jason Potas]

Hi everyone/anyone,

I would like to store frozen rat spinal cord sections in a -20 degree C
freezer. Does anyone have experience with the following method, ie does it
work:

1-cut sections frozen onto gelatinized glass slide.
2-air dry overnight at room temperature (to remove all moisture)
3-wrap multiple glass slides inside aluminium foil together like a pack of
chewing gum
4-store in the freezer at -20 deg C
5-when required, removed one (or required number) slide(s) from the pack,
but without defrosting other slides
6-allow to reach room temperature and commence histochemistry as normal

does it work without stuffing up the slides or the tissue sticking on the
back of other slides. has someone done this?

cheers
jason


-- 
______________________________________
Jason Potas (PhD)
Laboratorio de Neurobiologia Celular e Molecular
Instituto de Biofisica Carlos Chagas Filho - IBCCF
Universidade Federal do Rio de Janeiro
CCS bloco J sala J1-029 Ilha do Fundao
21941-590 Rio de Janeiro, RJ - Brasil
Tel: +5521 2280 4694
      +5521 2562 6554
Email: jason <@t> biof.ufrj.br