[Histonet] RNA later

[Tony Henwood]

A quick rinse in water is good, followed by an hour or so infiltration
with OCT solution (about a 30-60% solution in water) should improve the
cryotomy.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
danarobinmarshal <@t> aol.com
Sent: Thursday, 10 August 2006 7:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: dkhabele <@t> mmc.edu
Subject: [Histonet] RNA later 


Hi everyone,
There was a thread earlier this year involving treatment of RNA-later
stored tissue such that it can be embedded and cut without fracturing.
I found one answer on the archives referring to a quick cold DEPC water
rinse prior to OCT.  Does anyone else have any advice as to how they
have handled this?
 
Thanks,
dana  
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