[Histonet] PFA v. formalin

Melissa Mazan melissa.mazan <@t> tufts.edu
Mon Aug 7 07:13:17 CDT 2006


How long can the tissues be left in the PFA (I realize it is not really 
PFA once in solution, so I suppose it should be called fresh formalin)? 
We're doing immunofluorescence using standard buffered formalin, some on 
archived tissues that have been sitting in formalin for several weeks. 
However, I keep hearing that freshly made formalin from PFA is 
preferable, but that it needs to be processed into wax blocks fairly 
rapidly? Thanks for any advice - Melissa

histonet-request <@t> lists.utsouthwestern.edu wrote:

> Send Histonet mailing list submissions to
> 	histonet <@t> lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
> 	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> 	histonet-request <@t> lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
> 	histonet-owner <@t> lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>    1. pfa vs. formalin (MVaughan4 <@t> ucok.edu)
>    2. Renal Necrosis (Linresearch <@t> aol.com)
>    3. Histology Jobs in your area (Eric Dye (ext 223))
>    4. HSV Controls (nick bowden)
>    5. Unsubscribe... (Cavett, Will)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Sat, 5 Aug 2006 16:57:31 -0500
> From: MVaughan4 <@t> ucok.edu
> Subject: [Histonet] pfa vs. formalin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<OF0DE7072B.F1E646F2-ON862571C1.007730A0-862571C1.00789FCF <@t> ucok.edu>
> Content-Type: text/plain; charset="US-ASCII"
> 
> Bryan,
> Molecular Probes sells various phallotoxin probes that lose effectiveness 
> binding to f-actin microfilaments when methanol is used as a fixative or 
> is part of a fixative (10% NBF). For these purposes I dilute 16% 
> formaldehyde, MeOH free, sold by Polysciences in sealed ampules, diluted 
> 1:1 with water, then that 8% mixture further diluted 1:1 with 0.2M 
> Phosphate buffer pH 7.4. It works great as a fixative.
> Mel
> 
> Melville B. Vaughan, Ph. D.
> Assistant Professor
> Department of Biology
> University of Central Oklahoma
> 100 N. University Drive
> Edmond, OK 73034
> http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
> 
> ------------------------------
> 
> Message: 2
> Date: Sat, 5 Aug 2006 18:58:37 EDT
> From: Linresearch <@t> aol.com
> Subject: [Histonet] Renal Necrosis
> To: histonet <@t> pathology.swmed.edu
> Message-ID: <417.80a33d7.32067c9d <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
> 
> Hello,
> Would anyone familiar with antibodies used for renal necrosis be willing to 
> share 
> source of Abs: RPA, GST, Clusterin and protocol?
> I am working with FFPE rat tissues.
> Lin
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 4 Aug 2006 14:03:51 -0400
> From: Eric Dye (ext 223) <Eric <@t> ategra.com>
> Subject: [Histonet] Histology Jobs in your area
> To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <QzBWVjg0SigmTCE3IVVgNTI4NTA3MjAz <@t> blkdell3l>
> Content-Type: text/plain
> 
> 
>    Hi - Fellow-Histonetters
> 
>    How are you?
>    Below  is  the  updated  list  of  Histology j o b s, All openings are
>    Dayshift  Monday  thru Friday unless indicated otherwise. All of these
>    jobs  are  looking to move quickly so if your interested call me A S A
>    P...
>    If  you  are  interested  in any of the Histology jobs listed below or
>    anywhere  else  -  please  call  me  at 800-466-9919 ext 223 or cell -
>    407-756-5507.
>    Most  HistoTech  j  o  b  s  are  permanent full-time unless indicated
>    otherwise
>    Here are some of my Newest Histology Jobs:
>    ------------------------------------------------------
>    Ohio (Southern) - perm - Bench Histotech
>    Washington, D.C - Histotech -perm and temp( 3-6 months)
>    New York (Long Island) - HistoTech - perm
>    Northern New Jersey - HistoTech and Cyto Tech - perm
>    Boston  Mass  -  HistoTech  -  one Senior HistoTech, One not so Senior
>    Histotech
>    Massachusetts (North of Boston) - perm - Bench Histotech
>    Minnesota   (Twin   Cities   area   -   perm   -   Bench  Histotech  -
>    Biotech/research - Make your own schedule!!!
>    Southeast Florida - perm - HistoTech
>    Southwest Florida - perm - Bench Histotech/ Supervisor
>    Florida, West Coast - both temp & perm openings- Bench Histotech
>    New  Hampshire  perm  openings - Bench Histotech (opportunity to learn
>    Mohs!)
> 
>    ---- end list of HistoTech Opportunities ---
>    If  you  are  interested  in  any of the Histology jobs listed above -
>    please call me at 800-466-9919 ext 223 or cell - 407-756-5507.
>    Thank you,
>    Eric Dye-Sr Allied Healthcare Recruiter
>    800-466-9919 ext 223 or Cell - 407-756-5507
>    P.S.: Feel free to pass along this email and my phone number to anyone
>    who you think might be interested.
>    P.S.S.:  The  clients  are  currently  interviewing - and the job will
>    close  soon  -  so  if  you  are  interested,  please call me today at
>    1-800-466-9919 ext 229
>    ---------------------------------------------------------------
>    Ategra Systems Inc
>    Specialists in Permanent & Contract Staffing
>    800 466 9919 ext 223 - voice
>    407 671 6075 - fax
>    [1]eric <@t> ategra.com
>    To Learn More About Ategra:
>    [2]http://www.ategra.com
>    ----------------------------------------------------------------
>    If you received this by mistake, or if you wish
>    not to hear from me, please shoot me a mail
>    to let me know and I'll not mail you again.
>    ----------------------------------------------------------------
>    ----------------------------------------------------------------
>    Note: this message is intended for:
>    Fellow-Histonetters at histonet <@t> lists.utsouthwestern.edu
>    ----------------------------------------------------------------
> 
> References
> 
>    1. mailto:eric <@t> ategra.com
>    2. http://www.ategra.com/
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Sun, 06 Aug 2006 20:42:17 +1200
> From: nick bowden <nickandmanda <@t> paradise.net.nz>
> Subject: [Histonet] HSV Controls
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20060806084218.A40D563CF05 <@t> smtp-2.paradise.net.nz>
> Content-Type: text/plain; charset=us-ascii
> 
> Hello!
> 
> Wow.. this is my very first time on histonet and I have a few technical
> things I would like to ask for help with but first is: Could anyone please
> tell me where I could purchase HSV I and II control sections for
> immunohistochemical staining?? Our previous supplier no longer supplies!!
>          Thanx, much appreciated!
>            Amanda 
>                
> 
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Sun, 6 Aug 2006 09:50:11 -0500
> From: "Cavett, Will" <WillCavett <@t> mhd.com>
> Subject: [Histonet] Unsubscribe...
> To: <Linresearch <@t> aol.com>,	<histonet <@t> pathology.swmed.edu>
> Message-ID: <293C7C19EFF7D611AE1A0002A53F81141BF1B929 <@t> omega.mhd.com>
> Content-Type: text/plain;	charset="us-ascii"
> 
> 
> Please unsubscribe me from this list.
> 
> ***********************************************************************
> 
> This electronic transmission contains information from Methodist Health
> System and should be considered confidential and privileged.  The
> information contained in the above messages is intended only for the
> use of the individual(s) and entity(ies) named above.  If you are not
> the intended recipient, be aware that any disclosure, copying,
> distribution, or use of this information is prohibited. If you receive
> this transmission in error, please notify the sender immediately by
> return e-mail.  Methodist Health System, its subsidiaries and
> affiliates hereby claim all applicable privileges related to the
> transmission of this communication.
> 
> 
> 
> ------------------------------
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> End of Histonet Digest, Vol 33, Issue 9
> ***************************************

-- 
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor of Large Animal Medicine
Director of Sports Medicine
Cummings School of Veterinary Medicine
Tufts University
200 Westborough Road
North Grafton, MA 01536
Tel: 508-839-5395
Fax: 508-839-7922
Email: melissa.mazan <@t> tufts.edu




More information about the Histonet mailing list