[Histonet] PFA v. formalin
Melissa Mazan
melissa.mazan <@t> tufts.edu
Mon Aug 7 07:13:17 CDT 2006
How long can the tissues be left in the PFA (I realize it is not really
PFA once in solution, so I suppose it should be called fresh formalin)?
We're doing immunofluorescence using standard buffered formalin, some on
archived tissues that have been sitting in formalin for several weeks.
However, I keep hearing that freshly made formalin from PFA is
preferable, but that it needs to be processed into wax blocks fairly
rapidly? Thanks for any advice - Melissa
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> 1. pfa vs. formalin (MVaughan4 <@t> ucok.edu)
> 2. Renal Necrosis (Linresearch <@t> aol.com)
> 3. Histology Jobs in your area (Eric Dye (ext 223))
> 4. HSV Controls (nick bowden)
> 5. Unsubscribe... (Cavett, Will)
>
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Sat, 5 Aug 2006 16:57:31 -0500
> From: MVaughan4 <@t> ucok.edu
> Subject: [Histonet] pfa vs. formalin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF0DE7072B.F1E646F2-ON862571C1.007730A0-862571C1.00789FCF <@t> ucok.edu>
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> Bryan,
> Molecular Probes sells various phallotoxin probes that lose effectiveness
> binding to f-actin microfilaments when methanol is used as a fixative or
> is part of a fixative (10% NBF). For these purposes I dilute 16%
> formaldehyde, MeOH free, sold by Polysciences in sealed ampules, diluted
> 1:1 with water, then that 8% mixture further diluted 1:1 with 0.2M
> Phosphate buffer pH 7.4. It works great as a fixative.
> Mel
>
> Melville B. Vaughan, Ph. D.
> Assistant Professor
> Department of Biology
> University of Central Oklahoma
> 100 N. University Drive
> Edmond, OK 73034
> http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
>
> ------------------------------
>
> Message: 2
> Date: Sat, 5 Aug 2006 18:58:37 EDT
> From: Linresearch <@t> aol.com
> Subject: [Histonet] Renal Necrosis
> To: histonet <@t> pathology.swmed.edu
> Message-ID: <417.80a33d7.32067c9d <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hello,
> Would anyone familiar with antibodies used for renal necrosis be willing to
> share
> source of Abs: RPA, GST, Clusterin and protocol?
> I am working with FFPE rat tissues.
> Lin
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 4 Aug 2006 14:03:51 -0400
> From: Eric Dye (ext 223) <Eric <@t> ategra.com>
> Subject: [Histonet] Histology Jobs in your area
> To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <QzBWVjg0SigmTCE3IVVgNTI4NTA3MjAz <@t> blkdell3l>
> Content-Type: text/plain
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>
> Hi - Fellow-Histonetters
>
> How are you?
> Below is the updated list of Histology j o b s, All openings are
> Dayshift Monday thru Friday unless indicated otherwise. All of these
> jobs are looking to move quickly so if your interested call me A S A
> P...
> If you are interested in any of the Histology jobs listed below or
> anywhere else - please call me at 800-466-9919 ext 223 or cell -
> 407-756-5507.
> Most HistoTech j o b s are permanent full-time unless indicated
> otherwise
> Here are some of my Newest Histology Jobs:
> ------------------------------------------------------
> Ohio (Southern) - perm - Bench Histotech
> Washington, D.C - Histotech -perm and temp( 3-6 months)
> New York (Long Island) - HistoTech - perm
> Northern New Jersey - HistoTech and Cyto Tech - perm
> Boston Mass - HistoTech - one Senior HistoTech, One not so Senior
> Histotech
> Massachusetts (North of Boston) - perm - Bench Histotech
> Minnesota (Twin Cities area - perm - Bench Histotech -
> Biotech/research - Make your own schedule!!!
> Southeast Florida - perm - HistoTech
> Southwest Florida - perm - Bench Histotech/ Supervisor
> Florida, West Coast - both temp & perm openings- Bench Histotech
> New Hampshire perm openings - Bench Histotech (opportunity to learn
> Mohs!)
>
> ---- end list of HistoTech Opportunities ---
> If you are interested in any of the Histology jobs listed above -
> please call me at 800-466-9919 ext 223 or cell - 407-756-5507.
> Thank you,
> Eric Dye-Sr Allied Healthcare Recruiter
> 800-466-9919 ext 223 or Cell - 407-756-5507
> P.S.: Feel free to pass along this email and my phone number to anyone
> who you think might be interested.
> P.S.S.: The clients are currently interviewing - and the job will
> close soon - so if you are interested, please call me today at
> 1-800-466-9919 ext 229
> ---------------------------------------------------------------
> Ategra Systems Inc
> Specialists in Permanent & Contract Staffing
> 800 466 9919 ext 223 - voice
> 407 671 6075 - fax
> [1]eric <@t> ategra.com
> To Learn More About Ategra:
> [2]http://www.ategra.com
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> References
>
> 1. mailto:eric <@t> ategra.com
> 2. http://www.ategra.com/
>
>
> ------------------------------
>
> Message: 4
> Date: Sun, 06 Aug 2006 20:42:17 +1200
> From: nick bowden <nickandmanda <@t> paradise.net.nz>
> Subject: [Histonet] HSV Controls
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20060806084218.A40D563CF05 <@t> smtp-2.paradise.net.nz>
> Content-Type: text/plain; charset=us-ascii
>
> Hello!
>
> Wow.. this is my very first time on histonet and I have a few technical
> things I would like to ask for help with but first is: Could anyone please
> tell me where I could purchase HSV I and II control sections for
> immunohistochemical staining?? Our previous supplier no longer supplies!!
> Thanx, much appreciated!
> Amanda
>
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Sun, 6 Aug 2006 09:50:11 -0500
> From: "Cavett, Will" <WillCavett <@t> mhd.com>
> Subject: [Histonet] Unsubscribe...
> To: <Linresearch <@t> aol.com>, <histonet <@t> pathology.swmed.edu>
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--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor of Large Animal Medicine
Director of Sports Medicine
Cummings School of Veterinary Medicine
Tufts University
200 Westborough Road
North Grafton, MA 01536
Tel: 508-839-5395
Fax: 508-839-7922
Email: melissa.mazan <@t> tufts.edu
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