[Histonet] pfa vs. formalin

MVaughan4 <@t> ucok.edu MVaughan4 <@t> ucok.edu
Sat Aug 5 16:57:31 CDT 2006

Molecular Probes sells various phallotoxin probes that lose effectiveness 
binding to f-actin microfilaments when methanol is used as a fixative or 
is part of a fixative (10% NBF). For these purposes I dilute 16% 
formaldehyde, MeOH free, sold by Polysciences in sealed ampules, diluted 
1:1 with water, then that 8% mixture further diluted 1:1 with 0.2M 
Phosphate buffer pH 7.4. It works great as a fixative.

Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034

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