[Histonet] VIP 5
Madary, Joseph
MadaryJ <@t> MedImmune.com
Wed Aug 2 12:42:01 CDT 2006
Just got it a couple of months ago and so far I cannot complain too
much. I have a couple of items I think are a bit backwards like I
cannot enter in how many cassettes I ran, I can only print out run info
if I want, I cannot turn off the paraffin if I want to save heating it
for a week if I am not running anything. I know that if you do not
stick with the maintenance it is funny, especially the warm water
flushes. Honestly I like what comes out at the end but I expected a
much more advanced product in terms of software. I would buy another
though.... I was using a dip and dunk so anything was better than that
Thermo Shandon Citadel 1000. I mean it is so unhealthy to have those
around, they should not be on the market. What a difference in the lab
fumes when we switched. I mean I used a dip and dunk in 1979, and could
not believe it when I got here and had to use one for a couple of years.
All good now though. Okay, probably in trouble with someone now.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, August 02, 2006 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 33, Issue 2
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Today's Topics:
1. RE: Foxp3 on Murine tissue (Melissa Gonzalez)
2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk))
3. Staining cultured cells (Pixley, Sarah (pixleysk))
4. immunohistochemical analysis for connective tissue (Xilong Li)
5. ImmunoVision Technologies, Co. (Helen E Johnson)
6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist)
7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum)
8. RE: Ethanol/other fixations for frozen sections (Tony Henwood)
9. Looking for Florida histotechs--or people who are interested
in Florida relocation: we help with the license. (Cheryl)
10. article from J of histotechnology (louise renton)
11. Zinc-Based Fixative (Shirley PHUA)
12. prefilled formalin containers (Vickroy, Jim)
13. Re: prefilled formalin containers (Jackie M O'Connor)
14. '07 TSH Meeting (Ford Royer)
15. The New VIP (Santana, Diane)
16. Re: The New VIP (Fred Underwood)
17. RE: The New VIP. . (Henry, Charlene)
18. Re: The New VIP (crains <@t> wpmpath.com)
19. prefilled formalin containers (Stahl, Michael)
20. Re: The New VIP (Andrea Grantham)
21. charging and CPT questions (Vickroy, Jim)
22. please unsubscribe me from the list (Jayne Scoggin)
23. RE: charging and CPT questions (Zajic Kari)
----------------------------------------------------------------------
Message: 1
Date: Tue, 1 Aug 2006 10:18:52 -0700
From: "Melissa Gonzalez" <Melissa.Gonzalez <@t> cellgenesys.com>
Subject: [Histonet] RE: Foxp3 on Murine tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A6B0506C2B000E45A7556B9A93C455475CAE3B <@t> hqsvr01mail.cgi.com>
Content-Type: text/plain; charset="us-ascii"
I've also tried several of the products out there with no success on
murine tissues as well. I would be interested in hearing if anyone has
had success with FoxP3 yet on murine tissues, and what you could use as
a positive control.
Thanks,
Melissa
------------------------------
Message: 7
Date: Mon, 31 Jul 2006 14:02:03 -0500
From: "Drew Allan Roenneburg" <ROENN <@t> surgery.wisc.edu>
Subject: [Histonet] Foxp3 on Murine tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <44CE0D5B0200009C00001F23 <@t> gw.surgery.wisc.edu>
Content-Type: text/plain; charset=US-ASCII
Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE
mouse tissues (spleen). I have tried the Abcam rabbit anti-human foxp3
antibody that works on FFPE human and monkey (tonsil and spleen),
however have had little success on mouse. Thanks Drew Roenneburg
------------------------------
Message: 2
Date: Tue, 1 Aug 2006 13:45:30 -0400
From: "Pixley, Sarah \(pixleysk\)" <PIXLEYSK <@t> UCMAIL.UC.EDU>
Subject: [Histonet] RE:BrdU and GFP staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E7340445E0595A418B2F8DE0AE480E2007A35A42 <@t> ucmail6.ad.uc.edu>
Content-Type: text/plain; charset="us-ascii"
Dear Steve:
I would stain for GFP with an antibody to GFP and do labeling with DAB.
I recommend the Elite ABC kits. The DAB is moderately resistant to the
BrdU penetration protocols. Then, fix your tissues well with
paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a
system that labels with a fluorescent antibody. This will only work,
however, if your GFP is in different cells, or it is in the cytoplasm of
large cells that have no nuclear staining. The DAB can obscure the
secondary fluorescent labeling.
Good luck. Stay Cool back at you.
Sarah Pixley,
Ohio
Message: 2
Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] BrdU and GFP expression
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060731172241.65699.qmail <@t> web38215.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Good afternoon from WI,
We have several FFPE blocks from 2003 that express GFP. Our lead
scientist would like to perform Brdu IHC in the same slides hoping to
express both. As expected the AR removes the GFP green label. Has
anyone any experience expressing Brdu and GFP on the same slide.
Stay cool,
Steve
------------------------------
Message: 3
Date: Tue, 1 Aug 2006 13:49:31 -0400
From: "Pixley, Sarah \(pixleysk\)" <PIXLEYSK <@t> UCMAIL.UC.EDU>
Subject: [Histonet] Staining cultured cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E7340445E0595A418B2F8DE0AE480E2007A35A43 <@t> ucmail6.ad.uc.edu>
Content-Type: text/plain; charset="us-ascii"
To stain cultured cells we routinely fix with 4% paraformaldehyde for
15-20 mins. But if the antigen is sensitive to aldehydes, then we fix
with ice-cold 100% methanol for 5-10 mins. Your acetone technique for 30
mins is extremely harsh for cultured cells. If you want to continue with
it, I would fix with the acetone for only a few minutes and try that.
As for the other person who asked about staining cultured cells, we do
exactly the same ICC as on sections, although we usually are able to
dilute the primary antibodies more than with frozen tissue sections
(using paraformaldehyde perfused tissues).
Sarah Pixley
Ohio
Message: 4
Date: Mon, 31 Jul 2006 13:41:54 -0400
From: Christopher C Overend <christopher.overend <@t> huskymail.uconn.edu>
Subject: [Histonet] IFA staining problems
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90 <@t> huskymail.uconn.edu>
Content-Type: text/plain; charset=us-ascii
I am new to the technique of immunofluorescence, and have been doing
some work with it lately on cultured cells. Specifically, I have been
staining intracellular virus with very good results. However, when i try
to detect cellular proteins, I do not get any staining. The proceedure i
have been using consists of "fixation" with 90% acetone for 30 min, at
4degrees C. the rest of the process had been much like an ELISA, all
incubations have been for 30 min at 4degrees. The buffer i have been
using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the problem could be
from the acetone and suggested trying a glyoxal fixative. Can anyone
offer some insights, or if there is a different protocol i might have to
follow using a glyoxal fixative with tissue culture?
Thank you!
Chris
Christopher Overend
Ph.D Student
University of Connecticut
Department of Pathobiology and Veterinary Sciences Storrs, CT
Christopher.overend <@t> uconn.edu
------------------------------
Message: 4
Date: Tue, 01 Aug 2006 13:23:06 -0500
From: "Xilong Li" <Xilong.Li <@t> UTSouthwestern.edu>
Subject: [Histonet] immunohistochemical analysis for connective tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <44CF55BA020000F000004E06 <@t> swnw124.swmed.edu>
Content-Type: text/plain; charset=US-ASCII
Hi, All members,
I am looking for a consistent protocol to stain collagen of connective
tissues in muscle frozen sections by immunohistochemical analysis
including information for primary antibody and second antibody used in
the protocols.
Thanks in advance.
xilong li
Dr. Xilong Li
Hypertension Division, Internal Medicine University of Texas
Southwestern Medical Center
5323 Harry Hiness Blvd-J4.142
Dallas, TX 75390
Tel: 214-648-9966(L)
Fax: 214-648-7902
------------------------------
Message: 5
Date: Tue, 1 Aug 2006 14:24:37 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] ImmunoVision Technologies, Co.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFD5CF0F47.E75933CF-ON852571BD.0064AF32-852571BD.0065230A <@t> notes.health.
state.ny.us>
Content-Type: text/plain; charset=US-ASCII
Hi Histonetters,
Does anyone know if ImmunoVision Technologies, Co. is still in
business? No one answers the phone ( 650-992-7563).
Helen
(hej01 <@t> health.state.ny.us)
------------------------------
Message: 6
Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT)
From: Ron Lagerquist <spinquist <@t> yahoo.com>
Subject: Re: [Histonet] ImmunoVision Technologies, Co.
To: Helen E Johnson <hej01 <@t> health.state.ny.us>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060801183241.80617.qmail <@t> web56106.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
They were purchased by VisionBiosystems (800-753-7264)
http://www.vision-bio.com/media_releases.html
Ron
Helen E Johnson <hej01 <@t> health.state.ny.us> wrote:
Hi Histonetters,
Does anyone know if ImmunoVision Technologies, Co. is still in business?
No one answers the phone ( 650-992-7563).
Helen
(hej01 <@t> health.state.ny.us)
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------------------------------
Message: 7
Date: Tue, 01 Aug 2006 14:33:51 -0400
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: [Histonet] Pennsylvania Histology Meeting for October 2006
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.1.1.1.2.20060801142646.01a26d88 <@t> mail.vet.upenn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Good Afternoon All,
We have our web site up and partially up. We do have our program online
and have the program in the download file area for you to review and if
register. The program can be printed off. Just go to www.pahisto.org
and look for downloads in the top line. Click on and open or save. The
format is a Microsoft Word document. If you have a problem or would
need a pre-printing copy please let either me or Gloria Limetti know.
Gloria can be reached at glorialimetti <@t> yahoo.com or 412-647-8535.
Thanks for your patience and we will be updating the program with
vendors and other information as we near the meeting in October.
Best Regards,
Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr. CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348
Phone - 610-925-6278
Fax - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu
------------------------------
Message: 8
Date: Wed, 2 Aug 2006 10:49:46 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Ethanol/other fixations for frozen sections
To: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC55520127C6FF <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Urgent Frozen Sections for H&E - we fix in methanol
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
T. Hooper
Sent: Monday, 31 July 2006 10:45 PM
To: Histonet
Subject: [Histonet] Ethanol/other fixations for frozen sections
Just taking a survey .... What are everyone's thoughts on using
ethanol for frozen sections? I have a colleague who swears by it but
I am an acetone person myself.
Thoughts, comments, input on what is the best fix for frozen section
IHC?
Thanks!
--
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 9
Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT)
From: Cheryl <tkngflght <@t> yahoo.com>
Subject: [Histonet] Looking for Florida histotechs--or people who are
interested in Florida relocation: we help with the license.
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20060802024034.14424.qmail <@t> web50904.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello everyone--
Florida's registry process has created a major shortage of qualified
techs in the state. We have a number of Florida permanent and temp
opportunities. The permanent labs will consider transitional job
situations to allow you to work while your license is processed. We
also have a number of temp situations for a great pay scale for techs
registered to work in the state. Travel isn't for everyone, but these
are good labs and a great way to start.
Either way, we'll help you through the process to get you through it
as quickly as possible. If you're even curious, give us a call. Of
course we have a number of other opportunities including sales and
management all over the country. We won't work with every lab--those we
place find themselves in jobs that fit--where they are happy to go to
work. We're here to help and no fee to you.
Tiffany is in the office and I'm traveling as a temp for a couple of
weeks--as always, referral bonuses are available--share us with your
friends!
Tiffany/Office 281.852.9457
Cheryl/Cell 281.883.7704
or this number follows me around: 800.756.3309 (it will ring a long
time to find me--please be patient and leave your phone number!)
We return all calls.
Thanks!
Cheryl
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one Tech at a time.
281.883.7704 c
281.852.9457 o
admin <@t> fullstaff.org
Sign up for the FREE monthly newsletter AP News--updates, tricks of the
trade and current issues for Anatomic Pathology Clinical Labs. Send a
'subscribe' request to APNews <@t> fullstaff.org. Please include your name
and specialty in the body of the email.
------------------------------
Message: 10
Date: Wed, 2 Aug 2006 09:47:06 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: [Histonet] article from J of histotechnology
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0608020047v2e62c7b9u9a6284948826acae <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi can anyone share a copy of this article? I would be very grateful
"Entering the realm of mineralized bone processing: a review of the
literature and techniques." J Histotechnol 1997:20 (3) p259-266
Many thanks
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 11
Date: Wed, 2 Aug 2006 16:19:02 +0800
From: Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg>
Subject: [Histonet] Zinc-Based Fixative
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF3FBA6707.3B4F8EC6-ON482571BE.002C8BD9-482571BE.002DAEB0 <@t> gems2.gov.sg>
Content-Type: text/plain; charset="US-ASCII"
Dear All,
Reference Article:
Lab Invest 2003 Jun;83(6):889-99
Zinc-based fixative improves preservation of genomic DNA and proteins in
histoprocessing of human tissues
Anyone out there know of the exact composition of any zinc-based
fixative
that is based on the following?
1. 0.5% Zinc Chloride and
2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium
acetate
Many thanks & regards,
Shirley Phua
Histopathology Laboratory
Centre for Forensic Medicine
Health Sciences Authority
Singapore
------------------------------
Message: 12
Date: Wed, 2 Aug 2006 08:23:30 -0500
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] prefilled formalin containers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A2AD4F58AD06B44FBA71D4B1C4B9571A0680A390 <@t> mmcmail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
We have experienced problems with prefilled formalin containers that we
send to our clients. We have tried two different vendors and they both
leak. One of the container types claims to have an o-ring type lid and
the other does not have an o-ring. The containers with the o-ring seems
to leak as much as the others. In fact the o-ring type sometimes leak
when they are received from the vendor. If anyone has dealt with this
successfully please let me know how you solved it.
Jim Vickroy
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
This message (including any attachments) contains confidential
information intended for a
specific individual and purpose, and is protected by law. If you are not
the intended recipient,
you should delete this message. Any disclosure, copying, or distribution
of this message, or the
taking of any action based on it, is strictly prohibited.
------------------------------
Message: 13
Date: Wed, 2 Aug 2006 08:41:32 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] prefilled formalin containers
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFFB622EF1.746166AF-ON862571BE.004AF8E5-862571BE.004B3FD8 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Are they leaking on the return trip? Hard to get your clients to make
sure they are screwed on properly. Each vial/jar should be contained
within a secondary leakproof bag, anyway - to prevent leaks in transit.
Give Surgipath a call.
Jackie O'
"Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/02/2006 08:23 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] prefilled formalin containers
We have experienced problems with prefilled formalin containers that we
send to our clients. We have tried two different vendors and they both
leak. One of the container types claims to have an o-ring type lid and
the other does not have an o-ring. The containers with the o-ring seems
to leak as much as the others. In fact the o-ring type sometimes leak
when they are received from the vendor. If anyone has dealt with this
successfully please let me know how you solved it.
Jim Vickroy
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
This message (including any attachments) contains confidential
information
intended for a
specific individual and purpose, and is protected by law. If you are not
the intended recipient,
you should delete this message. Any disclosure, copying, or distribution
of this message, or the
taking of any action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 2 Aug 2006 08:42:33 -0500
From: "Ford Royer" <froyer <@t> bitstream.net>
Subject: [Histonet] '07 TSH Meeting
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003101c6b639$82553090$7701a80a <@t> Ford>
Content-Type: text/plain; charset="us-ascii"
Would the person(s) who are organizing next years Texas Society of
Histotechnology meeting please contact me?
Thanks!
~ Ford
Ford M. Royer, MT(ASCP)
Histology Product Manager
Minnesota Medical, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
CELL: 612-839-1046
Phone: 763-542-8725
Fax: 763-546-4830
eMail: froyer <@t> bitstream.net
------------------------------
Message: 15
Date: Wed, 2 Aug 2006 10:05:40 -0400
From: "Santana, Diane" <dsantana <@t> pmaonline.com>
Subject: [Histonet] The New VIP
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5 <@t> MAILPMA>
Content-Type: text/plain; charset="iso-8859-1"
I have a question and please nobody get all defensive about this, but
has
anybody recently bought the VIP 5 tissue processor? If you have, do or
did
you have any problems with it?
Thanks
Diane Santana
PMA
Haverhill, Mass.
------------------------------
Message: 16
Date: Wed, 02 Aug 2006 10:31:26 -0400
From: "Fred Underwood" <funderwood <@t> mcohio.org>
Subject: Re: [Histonet] The New VIP
To: <histonet <@t> lists.utsouthwestern.edu>, <dsantana <@t> pmaonline.com>
Message-ID: <s4d07f0a.084 <@t> mcohio.org>
Content-Type: text/plain; charset=US-ASCII
I've had mine for a year and (knock, knock on wood) have had no
problems.
Fred
>>> "Santana, Diane" <dsantana <@t> pmaonline.com> 08/02 10:05 AM >>>
I have a question and please nobody get all defensive about this, but
has
anybody recently bought the VIP 5 tissue processor? If you have, do or
did
you have any problems with it?
Thanks
Diane Santana
PMA
Haverhill, Mass.
_______________________________________________
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------------------------------
Message: 17
Date: Wed, 2 Aug 2006 09:36:48 -0500
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
Subject: RE: [Histonet] The New VIP. .
To: "Santana, Diane" <dsantana <@t> pmaonline.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5CB39BCA5724F349BCB748675C6CA1A2099A1D6A <@t> SJMEMXMB02.stjude.sjcrh.local>
Content-Type: text/plain; charset="US-ASCII"
We purchased a VIP5 a few years ago and had several problems so it was
replaced with a new VIP5. Since that time we have had no problems and it
has been a reliable instrument.
Charlene
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Santana,
Diane
Sent: Wednesday, August 02, 2006 9:06 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] The New VIP. .
I have a question and please nobody get all defensive about this, but
has anybody recently bought the VIP 5 tissue processor? If you have, do
or did you have any problems with it?
Thanks
Diane Santana
PMA
Haverhill, Mass.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Wed, 2 Aug 2006 10:30:16 -0500
From: <crains <@t> wpmpath.com>
Subject: Re: [Histonet] The New VIP
To: "Santana, Diane" <dsantana <@t> pmaonline.com>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<20060802153018.PMMJ26069.dukecmmtao03.coxmail.com <@t> dukecmmtao03>
Content-Type: text/plain; charset=ISO-8859-1
We have a VIP 5 that we purchased a little over a year ago. Have not
had a single problem with it so far.
Chris Rains
WPM Pathology Laboratory
Salina, KS
>
> From: "Santana, Diane" <dsantana <@t> pmaonline.com>
> Date: 2006/08/02 Wed AM 09:05:40 CDT
> To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] The New VIP
>
> I have a question and please nobody get all defensive about this, but
has
> anybody recently bought the VIP 5 tissue processor? If you have, do or
did
> you have any problems with it?
> Thanks
> Diane Santana
> PMA
> Haverhill, Mass.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Important: This email and any attachments may contain confidential
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Privacy of Individually Identifiable Health Information (45 C.F.R. Parts
160 and 164). If you or your organization is a "Covered Entity" under
the above mentioned regulations, you are obligated to treat such
information in a manner consistent with the regulations. If it appears
that this email was sent to you in error, (1) you are prohibited from
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appreciate your cooperation.
------------------------------
Message: 19
Date: Wed, 2 Aug 2006 11:42:17 -0400
From: "Stahl, Michael" <mstahl <@t> bvha.org>
Subject: [Histonet] prefilled formalin containers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4C878E714B21EB4F8EB159777B8822EE5B6834 <@t> bvfyms01.net.bvha.org>
Content-Type: text/plain; charset="us-ascii"
We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We
found these to be almost leak proof. However, they do leak when the jars
arnt screwed on correctly. But we agree, its not pleasant to have a
specimen jar in a bio hazard bag full of fixative. Just try to find that
cervial biospy!
Michael P. Stahl HT (ASCP)
BVRHC
145 W Wallace St
Findlay, OH 45840
mstahl <@t> bhva.org
------------------------------
Message: 20
Date: Wed, 02 Aug 2006 08:55:45 -0700
From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: Re: [Histonet] The New VIP
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4.3.2.7.2.20060802085142.00cec460 <@t> algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I just got a new VIP 5 and I love it. Wanted one all my (histotech) life
and I'm so happy to finally have it! It has been great. So easy to use
and
the tissues are coming out wonderful. Knock-on-wood it will do as well
as
the old Tissue Tek dipper/dunker that it is replacing.
Andi
At 10:30 AM 8/2/2006 -0500, crains <@t> wpmpath.com wrote:
>We have a VIP 5 that we purchased a little over a year ago. Have not
had
>a single problem with it so far.
>
>Chris Rains
>WPM Pathology Laboratory
>Salina, KS
> >
> > From: "Santana, Diane" <dsantana <@t> pmaonline.com>
> > Date: 2006/08/02 Wed AM 09:05:40 CDT
> > To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> > Subject: [Histonet] The New VIP
> >
> > I have a question and please nobody get all defensive about this,
but has
> > anybody recently bought the VIP 5 tissue processor? If you have, do
or did
> > you have any problems with it?
> > Thanks
> > Diane Santana
> > PMA
> > Haverhill, Mass.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>Important: This email and any attachments may contain confidential
>information subject to protection under the Federal Standards for
Privacy
>of Individually Identifiable Health Information (45 C.F.R. Parts 160
and
>164). If you or your organization is a "Covered Entity" under the
above
>mentioned regulations, you are obligated to treat such information in a
>manner consistent with the regulations. If it appears that this email
was
>sent to you in error, (1) you are prohibited from utilizing or
>disseminating this email or any attachments; (2) please immediately
delete
>it from your computer and any servers or other locations where it might
be
>stored and email (crains <@t> wpmpath.com) or call (Chris Rains) at
>785-823-7201 advising that you have done so. We appreciate your
cooperation.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth <@t> u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
------------------------------
Message: 21
Date: Wed, 2 Aug 2006 11:08:00 -0500
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] charging and CPT questions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A2AD4F58AD06B44FBA71D4B1C4B9571A0680A395 <@t> mmcmail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
Can anyone shed any light on the proper way to code and charge the
following:
1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319?
I'm not sure which one.
2. Touch preps - Done when we do a frozen section. The frozen
section is 88331 and the touch prep should be 88333 or 88334?
3. Semi-thin sectioning of nerve biopsies - Plastic embedding
In the past we charged all nerve bxs with an 88348 (EM) charge since
not only did we do the semi-thin sectioning but we
also did the transmission EM. Our neuropathologist now wants the
semi-thin sections
routinely but not the rest of the EM examination.
Thanks
Jim Vickroy
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
This message (including any attachments) contains confidential
information intended for a
specific individual and purpose, and is protected by law. If you are not
the intended recipient,
you should delete this message. Any disclosure, copying, or distribution
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taking of any action based on it, is strictly prohibited.
------------------------------
Message: 22
Date: Wed, 2 Aug 2006 09:45:21 -0700
From: "Jayne Scoggin" <jscoggin <@t> biocept.com>
Subject: [Histonet] please unsubscribe me from the list
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200608021624.k72GO3Vh004624 <@t> mx.bioceptlabs.com>
Content-Type: text/plain; charset="us-ascii"
Please unsubscribe me from the list.
Jayne Scoggin, Biocept Inc.
858 320 8224
jscoggin <@t> biocept.com
------------------------------
Message: 23
Date: Wed, 2 Aug 2006 12:40:55 -0400
From: "Zajic Kari" <Kari.Zajic <@t> HCAhealthcare.com>
Subject: RE: [Histonet] charging and CPT questions
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<095327C7CDBDF64B9E9728A54799091E015CC5A2 <@t> ORLEV03.hca.corpad.net>
Content-Type: text/plain; charset="iso-8859-1"
Hi Jim. I do not do muscle or nerve at my facility but we send to the
University of Miami and they charge the following:
Muscle
88305 x1: Surgical Path,gross,micro
88313 x2: Paraffin: H&E Plastic
88314 x2: cryostat H&E Trichrome
88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH
Nerve
88305 x1: Surg Path,gross,micro
88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal
Violet,Silver Stain
88314 x2: Cryostat: H&E,Trichrome
88319 x1: Frozen Section Histochem: Esterase
88348 x1: Nerve Electron Micro
88356 x1: Nerve Morphometry
88362 x1: Nerve Teased Preps
As far as frozen section touch preps, they came out with new CPT's in
2006 as follows:
88333 Path consult during surgery (cyto exam) initial site ie.touch
prep,squash prep
physician fee global $75-100+
technical $17-25
88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep
physicain fee $40-50+
technical $10-15
this info was given to me by Health Systems Concepts,Inc.
hope this helps!!!
Kari :)
Kari Marie Zajic HT,MLT
Histology Supervisor
Palms West Hospital
Pathology Department
13001 State Road Eighty
Loxahatchee, Florida 33470
phone: (561)798-6036
telefax: (561)753-4298
voicemail: (561)753-4299
pager: (561)610-4949
email: Kari.Zajic <@t> HCAHealthcare.com
This email and any files transmitted with it may contain PRIVILEGED or
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Vickroy,
Jim
Sent: Wednesday, August 02, 2006 12:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] charging and CPT questions
Can anyone shed any light on the proper way to code and charge the
following:
1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319?
I'm not sure which one.
2. Touch preps - Done when we do a frozen section. The frozen
section is 88331 and the touch prep should be 88333 or 88334?
3. Semi-thin sectioning of nerve biopsies - Plastic embedding
In the past we charged all nerve bxs with an 88348 (EM) charge since
not only did we do the semi-thin sectioning but we
also did the transmission EM. Our neuropathologist now wants the
semi-thin sections
routinely but not the rest of the EM examination.
Thanks
Jim Vickroy
Technical Supervisor - Surgical and Autopsy Pathology
Memorial Medical Center
This message (including any attachments) contains confidential
information intended for a
specific individual and purpose, and is protected by law. If you are not
the intended recipient,
you should delete this message. Any disclosure, copying, or distribution
of this message, or the
taking of any action based on it, is strictly prohibited.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 33, Issue 2
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