[Histonet] Protocol for MOM kit ( whole mount staining)

[sohail ejaz]

I am trying whole mount staining of mouse Retina, Trachea and 20 micrometer tissues with vWF (Rabbit anti human von Willibrand factor polyclonal antibody, Chemicon international) SMA (Monoclonal anti-actin, alpha smooth muscle antibody produced in mouse) and i need to use MOM kit (Vector) for this purpose.
   
  They recommend to incubate the samples for just 30 min with primary antibody.
  I wonder if this time is enough..................?? 
  Can anyone guide me a good protocol regarding this issue.
   
  Thanks
   
  Dr. Sohail

		
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