[Histonet] RE: Bone marrow FE stains

[Disher Lori]

Laura,

I make two drip slides when assisting in doing a bone marrow.  After they dry we do an FE stain on one of them.  If the drip is FE positive (as long as it is a good strong positive) I will save the 2nd drip slide for a control and we do not have to do the FE on the bx and clot the next day.
Hope this helps.
Lori Disher
lori.disher <@t> hcahealthcare.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, April 20, 2006 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 29, Issue 23


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Today's Topics:

   1. dvaneyck IHCRG Membership Application Form (Patsy Ruegg)
   2. RE: Expiry Period - Immunofluorescence Dilutions (Gagnon, Eric)
   3. histogel cryosections (Steven Coakley)
   4. Expiry Period  - Immunofluorescence Dilutions (Gagnon,	Eric)
      (Emma JONES)
   5. RE: dvaneyck IHCRG Membership Application Form (Patsy Ruegg)
   6. bone marrow Fe stains (Galiotto, Laura)
   7. Mouse Cytokeratins (Andrea T. Hooper)
   8. CBG Recycler (Gus Mondragon)
   9. RE: CD-3 woes (not any longer!) (C.M. van der Loos)
  10. RE: mouse spleen confocal (C.M. van der Loos)
  11. RE: Over lapped flouresence in double staining (C.M. van der Loos)
  12. Re: Large vessel endothelium problem (Mikael Niku)
  13. Diff Quick (Kemlo Rogerson)


----------------------------------------------------------------------

Message: 1
Date: Wed, 19 Apr 2006 12:48:50 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] dvaneyck IHCRG Membership Application Form
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <200604191848.k3JImnka016661 <@t> chip.viawest.net>
Content-Type: text/plain;	charset="us-ascii"

verify NSH membership please
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

  _____  

From: webmaster <@t> neo.agsci.colostate.edu
[mailto:webmaster <@t> neo.agsci.colostate.edu] 
Sent: Wednesday, April 19, 2006 11:30 AM
To: pruegg <@t> ihctech.net
Subject: IHCRG Membership Application Form


  _____  


Last_Name: Van Eyck
First_Name: Debra
NSH_Member: Yes
Employer: WAukesha Memorial Hospital
Address: 725 American Avenue
City: Waukesha
State: WI
Zip: 53188
Province: 
Country: USA
Phone: 262-928-2112
Ext: 
Fax: 262-928-4849
Email: deb.vaneyck <@t> phci.org
Surgical_Pathology: Yes
Hematopathology: 
Orthopedic: 
Veterinary: 
Immunology: 
Plastics: 
Research: 
Paraffin: Yes
Frozen: 
Cytospins: Yes
Smears_Touch_Preps: Yes
Plastics_sample: 
Sample_Other: 
PAP: 
APAAP: 
ABC_HRP: Yes
ABC_AP: Yes
SA_HRP: Yes
SA_AP: Yes
IF: 
IHC_Other: 
ISH: 
Gels: 
PCR: 
Mol_Other: 
Automated_IHC: Yes
Company: Dako
IHC_Qualification: No
Date_Taken_Exam: 
Like_To_Take_Exam: Yes
Expected_Date: next 6 months
Need_Tissues: No
Tissue_Needed: 
Need_Reagents: No
Reagents_Needed: 
Can_Provide_Tissues: No
Tissues_Provided: 
Can_Provide_Reagents: No
Reagents_Provided: 
Form: Submit
Remote Name: 207.67.109.90
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1;
.NET CLR 1.1.4322)



Other_Tech




------------------------------

Message: 2
Date: Wed, 19 Apr 2006 15:28:04 -0400
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] RE: Expiry Period - Immunofluorescence Dilutions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F93BD6329FC3AE4C8DB116B985FBC3130972067E <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain;	charset="iso-8859-1"

Cynthia, Glen and Gudrun, thanks for your input on making up extending the life of antisera-FITC dilutions for our immunofluorescence.  Excellent answers, much appreciated.  
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada
  
 




------------------------------

Message: 3
Date: Wed, 19 Apr 2006 12:49:18 -0700 (PDT)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] histogel cryosections
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060419194918.72148.qmail <@t> web38208.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Has anyone ever used histogel/oct for FS.  I've been asked to attempt cryosectioning cultured embryo bodies fr IHC.
   
  Steve

			
---------------------------------
Celebrate Earth Day everyday!  Discover 10 things you can do to help slow climate change. Yahoo! Earth Day

------------------------------

Message: 4
Date: Wed, 19 Apr 2006 21:51:21 +0200
From: "Emma JONES" <EJones <@t> Ventanamed.com>
Subject: [Histonet] Expiry Period  - Immunofluorescence Dilutions
	(Gagnon,	Eric)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F132EB22FDA6744E929F204E7B5B21BC012D9835 <@t> PROSPER.VENTANA.VENTANAMED.COM>
	
Content-Type: text/plain;	charset="windows-1250"

Hi Eric, 
Have you considered asking your Ventana rep for information on their FITC reagents. The expiry dates on those are far longer than any you will get from making your own up. Approximately 12 months +
regards

Emma Jones

Today's Topics:

   1. CD-3 woes (Favara, Cynthia (NIH/NIAID) [E])
   2. RE: mouse spleen confocal (Favara, Cynthia (NIH/NIAID) [E])
   3. Expiry Period  - Immunofluorescence Dilutions (Gagnon, Eric)
   4. Re: mouse spleen confocal (Gayle Callis)
   5. RE: Expiry Period  - Immunofluorescence Dilutions
      (Favara, Cynthia (NIH/NIAID) [E])
   6. RE: Expiry Period  - Immunofluorescence Dilutions (Dawson, Glen)
   7. Re: lendrums' phloxine/tartrazine (John Kiernan)
   8. Re: mouse spleen confocal (Scott Parker)
   9. RE: RE: RHS-1 and MicroMed T/T Mega/Jeanine (Joyce Cline)
  10. Re- Unscri be (Quadeer, Shahnaz S.)
  11. IEC CTD harris cryostat (dfs dsaf)
  12. Over lapped flouresence in double staining (sohail ejaz)
  13. RE: Cryostat (Molinari, Betsy)
  14. beginner questions on methacrylate resins (Nancy W. Troiano)
  15. M. leprae control slides (Bartlett, Jeanine)
  16. Microwave transparent plastics: Coleman Mfg contact
      information (Cheryl)
  17. Bouin's alternative/substitution question (jason.burrill <@t> crl.com)
  18. Re: Mouse lungs OCT and curling (Gayle Callis)


----------------------------------------------------------------------


 



Message: 3
Date: Tue, 18 Apr 2006 13:23:28 -0400
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] Expiry Period  - Immunofluorescence Dilutions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F93BD6329FC3AE4C8DB116B985FBC31309720673 <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain;	charset="iso-8859-1"

 
 
Hello Listers,
 
We currently do immunofluorescence for IgG, IgA, IgM, C3, C1q, Fib., Kappa, Lambda, and C4d on renal biopsies, and the first four on skin biopsies.  We are using Dako Antibody Diluting Buffer and mostly Dako FITC antisera.
 
We currently make up about 1 ml of each, and the dilutions last us between 1 and 3 weeks.  We would like to keep them longer if possible, making them up in larger quantities, possibly with the goal of transferring them to dispensers on our Ventana XT's.
]
Is there anyone doing the same immunofluorescence panel and dilutions, and if so, how long are you able to use your dilutions once made?
 
Thanks for your assistance,
 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada





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------------------------------

Message: 5
Date: Wed, 19 Apr 2006 13:53:46 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] dvaneyck IHCRG Membership Application Form
To: "'Patsy Ruegg'" <pruegg <@t> ihctech.net>,
	<histonet <@t> pathology.swmed.edu>
Message-ID: <200604191953.k3JJrjka001675 <@t> chip.viawest.net>
Content-Type: text/plain;	charset="us-ascii"

Sorry, I meant to send this to NSH, it comes up as histo on my server and I
hit the wrong button.  Disregard this message to verify NSH membership to
HISTONET
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Wednesday, April 19, 2006 11:49 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] dvaneyck IHCRG Membership Application Form

verify NSH membership please
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

  _____  

From: webmaster <@t> neo.agsci.colostate.edu
[mailto:webmaster <@t> neo.agsci.colostate.edu]
Sent: Wednesday, April 19, 2006 11:30 AM
To: pruegg <@t> ihctech.net
Subject: IHCRG Membership Application Form


  _____  


Last_Name: Van Eyck
First_Name: Debra
NSH_Member: Yes
Employer: WAukesha Memorial Hospital
Address: 725 American Avenue
City: Waukesha
State: WI
Zip: 53188
Province: 
Country: USA
Phone: 262-928-2112
Ext: 
Fax: 262-928-4849
Email: deb.vaneyck <@t> phci.org
Surgical_Pathology: Yes
Hematopathology: 
Orthopedic: 
Veterinary: 
Immunology: 
Plastics: 
Research: 
Paraffin: Yes
Frozen: 
Cytospins: Yes
Smears_Touch_Preps: Yes
Plastics_sample: 
Sample_Other: 
PAP: 
APAAP: 
ABC_HRP: Yes
ABC_AP: Yes
SA_HRP: Yes
SA_AP: Yes
IF: 
IHC_Other: 
ISH: 
Gels: 
PCR: 
Mol_Other: 
Automated_IHC: Yes
Company: Dako
IHC_Qualification: No
Date_Taken_Exam: 
Like_To_Take_Exam: Yes
Expected_Date: next 6 months
Need_Tissues: No
Tissue_Needed: 
Need_Reagents: No
Reagents_Needed: 
Can_Provide_Tissues: No
Tissues_Provided: 
Can_Provide_Reagents: No
Reagents_Provided: 
Form: Submit
Remote Name: 207.67.109.90
HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1;
.NET CLR 1.1.4322)



Other_Tech


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 6
Date: Wed, 19 Apr 2006 14:58:11 -0500
From: "Galiotto, Laura" <LGaliotto <@t> nch.org>
Subject: [Histonet] bone marrow Fe stains
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<270614B321ACB44D8C1D91F4F921FDC3036CBCBE <@t> NCH01EX02.nch.org>
Content-Type: text/plain;	charset="iso-8859-1"

I have a problem with bone marrow aspirate controls. The problem is that we are limited to the amount of controls we can collect.  Even though the tissue control is working if the aspirate control (which are difficult to obtain) does not work we are being asked to repeat the stain until we find a aspirate control that is acceptable. Since I can not guarantee the aspirate control contains an adequate amount of Fe, I will be wasting time and reagents. 
Can anyone give feedback on how they process stains on bone marrow's, do you only use a tissue control, does anyone know where bone marrow aspirate controls -positive for Iron -can be purchased commercially? 

Laura Galiotto, HT (ASCP)
Histology Facilitator

Northwest Community Hospital 
800 West Central Road
Arlington Heights, Il 60005
847-618-6190



------------------------------

Message: 7
Date: Wed, 19 Apr 2006 19:56:24 -0400
From: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
Subject: [Histonet] Mouse Cytokeratins
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <p06200706c06c7e65ed88@[140.251.51.71]>
Content-Type: text/plain; charset=us-ascii; format=flowed

Hi All,

What antibodies are people using to stain for cytokeratins in mouse 
tissue - frozen or paraffin.

Thanks in advance,
Andrea
-- 



------------------------------

Message: 8
Date: Thu, 20 Apr 2006 02:18:58 -0400
From: "Gus Mondragon" <gmondragon <@t> gsopath.com>
Subject: [Histonet] CBG Recycler
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<03B63DE44B5C014D84F9A9D8A968B5174C4D60 <@t> lithium.corp.gsopath.com>
Content-Type: text/plain;	charset="us-ascii"

Hello Histonetters; I have a CBG 5 gal. Alcohol or Xylene recycler for
sale that we no longer use. Good efficient unit is 4 yrs old in
excellent condition, trouble/maintenance free. I'd be glad to offer good
suggestions on recycling program. If you have any available Leica
electric or manual microtomes, let's talk. Gus Mondragon (336) 387-2536;
Greensboro NC


------------------------------

Message: 9
Date: Thu, 20 Apr 2006 08:51:11 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: CD-3 woes (not any longer!)
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3263403287ca.3287ca326340 <@t> amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"


   Cynthia,

   We  were  not that successful either with the Dako rabbit CD3 antibody
   on  mouse  tissue.  We  changed to the LabVision CD3 rabbit monoclonal
   (clone  SP7) and this worked very well on human, rat and mouse tissues
   (both  cryo  and  FFPE).  E.g.  for staining FFPE: HIER with Tris-EDTA
   pH9.0,  CD3 1:1000 (60 min, RT), polymer anti-rabbit/HRP (30 min, RT),
   DAB+ (5 min, RT).

   Hope this helps,

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   Date: Tue, 18 Apr 2006 13:15:00 -0400
   From: "Favara, Cynthia \(NIH/NIAID\) [E]" <cfavara <@t> niaid.nih.gov>
   Subject: [Histonet] CD-3 woes
   To: <Histonet <@t> lists.utsouthwestern.edu>
   I have been using Dako CD-3 on murine formalin fixed paraffin embedded
   tissue and am experiencing some variation in staining. My protocol:
   pretreatment  with  Citrate pH 6.0 @120C for 5 minutes under pressure,
   an
   overnight incubation of the primary 1:1500 @4C, followed by 30 minute
   incubation with Vector biotinylated anti Rabbit IgG 1:250, 30 minutes
   Biogenex SS Streptavidin all steps following primary are done on the
   Ventana Nexus @RT using their AEC. I used to do the stain reliably but
   have  a  new  lot and reliability is not optimal. I use a mouse spleen
   and
   brain  with T-cell infiltration as positive controls and a NL brain as
   a
   negative  control.  Sometimes the stain is so crisp and other times it
   is
   muddy with a decrease in the number of cells st! ained. I


------------------------------

Message: 10
Date: Thu, 20 Apr 2006 09:05:26 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: mouse spleen confocal
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3294ac32b9ea.32b9ea3294ac <@t> amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"


   Dear Scott,

   You  didn't  tell us what detection system was used. Your story sounds
   reminds   me   to   our   first  experiments  with  streptavidin-based
   detection on   mouse   spleen  when  we  were  confronted  with  heavy
   endogenous   biotin!   For   staining  mouse  spleen,  either  avoid a
   streptavidin-based detection   system   or   try  to  block endogenous
   biotin as Gayle suggested.

   Please  realize that if the above is true and/or Gayle's is right with
   her  suggestion  concerning  the  adsorption  of the anti-rat reagent,
   the signal  you observe is no autofluorescence but "unwanted" specific
   fluorescence!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   From: Scott Parker [[1]mailto:scott9379 <@t> gmail.com]
   Sent: Tuesday, April 18, 2006 9:05 AM
   To: histonet
   Subject: [Histonet] mouse spleen confocal
   Dear all,
   Can anybody help me with some antibody staining I have been doing on
   mouse
   spleen? We are trying to detect CD8 in 5 um sections using CD8b.2
   (ly-3.2) (
   53-5.8) with secondary Alexa Fluor 633 from mol. probes. Unfortunately
   all
   we see is vast amounts of auto-fluorescence making antibody detection
   impossible. We really need to get this sorted because ultimatley we'd
   like
   to  have  GFP  and  two  different  secondaries (labelling 3 things in
   total).
   Our protocol is basically:
   Spleens are removed to OCT and frozen in isopentane, sectioned (5 um)
   and
   stored at -20.
   When ready for use slides are warmed to room temp and fixed in -20 C
   acetone
   for 3 mins then re-hydrated in PBS for 5 mins.
   Secti! ons are a

References

   1. javascript:main.compose('new', 't=scott9379 <@t> gmail.com')


------------------------------

Message: 11
Date: Thu, 20 Apr 2006 09:16:29 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: Over lapped flouresence in double staining
To: histonet <@t> lists.utsouthwestern.edu
Cc: sohail_e <@t> yahoo.com
Message-ID: <50578550cc68.50cc68505785 <@t> amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"


   Dear Dr. Sohail,

   You  didn't  write  us  what detection system was used for this double
   staining.  The  fact  you observed overlap of SMA and vWF (which isn't
   very  likely  indeed) makes your detection system a bit suspect. To my
   opinion  the most straightforward (and safe) ways to doublestain these
   antibodies are:

   A:  cocktail  of  SMA  (mouse) + vWF (rabbit), followed by a secondary
   cocktail composed of goat anti-mouse/FLUO-1 + goat anti-rabbit/FLUO-2

   B: cocktail of SMA, clone 1A4 (mouse IgG2a) + vWF , clone F8/86 (mouse
   IgG1),  followed  by a secondary  cocktail composed of goat anti-mouse
   IgG2a/FLUO-1 + goat anti-mouse IgG1/FLUO-2

   Lots of success!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Tue, 18 Apr 2006 19:00:05 -0700 (PDT)
   From: sohail ejaz <sohail_e <@t> yahoo.com>
   Subject: [Histonet] Over lapped flouresence in double staining
   To: [1]histonet <@t> lists.utsouthwestern.edu

   I  am doing double staining of endothelial cells and smooth muscles of
   the blood vessels with vWF and SMA.

      While doing double staining I have encountered overlapping of  both
   antibodies  and  I  cant  see  a  clear  differentiation  between  the
   fluorescence of these two antibodies.

     Could you please guide me how to over come this problem.

     Thanks

     Dr. Sohail

References

   1. mailto:histonet <@t> lists.utsouthwestern.edu


------------------------------

Message: 12
Date: Thu, 20 Apr 2006 10:36:15 +0300
From: Mikael Niku <mikael.niku <@t> helsinki.fi>
Subject: Re: [Histonet] Large vessel endothelium problem
To: Gayle Callis <gcallis <@t> montana.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <444739EF.3020701 <@t> helsinki.fi>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hello again. Sorry for a bit unclearly formatted question.

More specifically, I'm doing samples of bovine blood vessels, obtained 
from a slaughterhouse. So perfusion is unfortunately out of question :)
And this is about REALLY big vessels, such as the bovine aorta. So the 
samples are bits (about 1 cm2) of vessel wall cut off and placed in PFA 
for overnight at +4C, then processed for paraffin sections in a routine 
manner.

And yes, this is primarily about blood vessels, not lymphatics.

Regards,
Mikael

Gayle Callis wrote:
> You did not indicate how you do the paraformaldehyde fixation?  
> Immersion or perfusion?
>
> Vascular perfusion followed by immersion for a few hours may help your 
> problem - to ensure the tissue is fixed from the inside out and in 
> particular the lumens of your large blood (?) vessels.
>
> You did not give details on what you do with the vessels (opened, etc, 
> stretched out) during fixation or processing? 


-- 
////////////////////////////////////////////////////////////

  Mikael Niku             URL: www.helsinki.fi/~mniku/
  University of Helsinki  Dept. Basic Veterinary Sciences

  - Mitäkö mieltä olen länsimaisesta sivistyksestä?
  Minusta se olisi erinomainen ajatus!                                                        
                                          - Gandhi

////////////////////////////////////////////////////////////




------------------------------

Message: 13
Date: Thu, 20 Apr 2006 14:17:57 +0100
From: Kemlo Rogerson <kemlo.rogerson <@t> waht.swest.nhs.uk>
Subject: [Histonet] Diff Quick
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<B4FA3DD12D42DA11A5DA00508BAF864902FDFCA4 <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain

We run a one-step FNAC breast clinic and have been using Diff-Quick as our
stain; I personally don't like it as I prefer Paps but there you are. We had
a recent FNAC that showed histiocytes in the tail of the prep but little
else. As you know the bubbly cells tend to migrate to the edges as they are
lighter but what we initially failed to notice was that in amongst the blood
were poorly stained clumps of cells. After restain with a 'proper'
Romanovsky stain they magically appeared and were benign. I shudder to think
what would have happened if they had not been spotted and had been
malignant. The problem I guess was the blood and together with the slow
drying of the smear.

Diff-Quick is fast but can have this effect, does anyone have a technique
that is nearly as fast as Diff Quick but more robust in these cases?

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 

 

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Tzu 

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End of Histonet Digest, Vol 29, Issue 23
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