[Histonet] re-freezing frozen sections

[dgaupp <@t> tulane.edu]

Histonet:

I have tissues(monkey brain) that were fixed(4%Paraformaldehyde),
cryprotected(20% sucrose), and flash froze(-70C isopentane).  Unfortunately,
mothernature(hurricane katrina) came threw and destroyed everything.  This
tissues sat for 3-4 months at blistering temps in the same position(melted OCT)
& some even had a surprise for me(fungus).  When the University let us come back
into our building, I rinsed off OCT and put the tissues in 10%NBF at room temp. 
The tissue was immersed for 3-4months in fixative.  I rinsed the tissue and once
again, flash froze one of the sections, and they cut horribly.  I don't know
what to do.  I am now tempting to paraffin process. I prefer to do frozens
because its easier to get almost every section of tissue(one sections I usually
get 500-700 slides).  As most of you know, brain likes to explode in water, so I
can do a better job if they are frozen.

Any help, I would appreciate!

Dina


Dina D. Gaupp, B.S., M.T.
Medical Research Specialist
Center for Gene Therapy
Tulane University Health Sciences Center
JBJ Bldg/Rm 658, SL-99
1430 Tulane Avenue
New Orleans, LA 70112
Lab: 504.988.1194
Fax: 504.988.7710
Email: dgaupp <@t> tulane.edu