[Histonet] Sudan Red 7B staining procedure

[Monfils, Paul]

I don't have an exact figure, but according to one of my older texts
(Histopathologic Technic and Practical Histochemistry by Lillie and
Fullmer), 4.25 grams Sudan Red 7B will dissolve in 100 ml absolute ethanol.
So the solubility in 70% ethanol should be substantially less than that.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Kim
> Merriam
> Sent: 	Tuesday, April 11, 2006 10:53 AM
> To: 	Histonet
> Subject: 	[Histonet] Sudan Red 7B staining procedure 
> 
> Hello,
>    
>   I have found a reference for staining fat with sudan red 7B, it calls
> for a "saturated" solution of sudan red 7B in 70% ethanol.  Does anyone
> know what the saturation point of sudan red 7B is in 70% ethanol; a
> general guide would be very helpful!
>    
>   Also - has anyone used this procedure before and what were the results
> compared to a regular Oil Red O (both in isopropanol and/or propylene
> glycol).
>    
>   Thanks in advance,
>   Kim
> 
> 
> Kim Merriam, MA, HT(ASCP)
> Novartis
> Cambridge, MA
> 			
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