[Histonet] What is your favorite frozen section fixative

[Bonner, Janet]

We use a mix of the two for FS fixative;  190cc 95% EtOH and  70cc NBF.

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Gayle Callis
Sent: Mon 4/10/2006 12:48 PM
To: Stephen Peters M.D.; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] What is your favorite frozen section fixative



What is the fixation for? H&E or another stain for rapid diagnostic purposes? 

For H&E frozen sections, 95% ethanol works fine but we also use neutral 
buffered formalin.  NBF, however, is not as FAST a fixative, but for 
morphology purposes, we like it better than 95% ethanol. 

For immunohistochemistry purposes, our fixation is MUCH different for 
frozen sections. 



At 08:31 AM 4/10/2006, you wrote: 
>HI histonetters, 
> 
>   I would like to do a comparison of the morphologic results using 
> different fixatives 
>    for frozen section slides. I have always been happy with 95% ETOH. I 
> know there are other good fixatives people use and I thought it would be 
> useful to compare them. I will photograph and add these results to my 
>   web site tutorial 
> (  http://pathologyinnovations.com/frozen_section_technique.htm ) and 
> frozen section lecture ( NSH Region 1 April 28 & 29 and California Society 
>   of Histotechnology May 18-21). 
>   Please let me know what you find is the best fixative for frozen 
> section morphology. 
> 
>   Stephen 
> 
> 
> 
> 
> 
>Stephen Peters M.D. 
>Vice Chairman of Pathology 
>Hackensack University Medical Center 
>201 996 4836 
> 
>Pathology Innovations, LLC 
>410 Old Mill Lane, 
>Wyckoff, NJ 07481 
>201 847 7600 
>www.pathologyinnovations.com 
> 
> 
> 
> 
>_______________________________________________ 
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Gayle Callis 
MT,HT,HTL(ASCP) 
Research Histopathology Supervisor 
Veterinary Molecular Biology 
Montana State University - Bozeman 
PO Box 173610 
Bozeman MT 59717-3610 
406 994-6367 
406 994-4303 (FAX) 



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