[Histonet] What is your favorite frozen section fixative

[Gayle Callis]

What is the fixation for? H&E or another stain for rapid diagnostic purposes?

For H&E frozen sections, 95% ethanol works fine but we also use neutral 
buffered formalin.  NBF, however, is not as FAST a fixative, but for 
morphology purposes, we like it better than 95% ethanol.

For immunohistochemistry purposes, our fixation is MUCH different for 
frozen sections.



At 08:31 AM 4/10/2006, you wrote:
>HI histonetters,
>
>   I would like to do a comparison of the morphologic results using 
> different fixatives
>    for frozen section slides. I have always been happy with 95% ETOH. I 
> know there are other good fixatives people use and I thought it would be 
> useful to compare them. I will photograph and add these results to my
>   web site tutorial 
> (  http://pathologyinnovations.com/frozen_section_technique.htm ) and 
> frozen section lecture ( NSH Region 1 April 28 & 29 and California Society
>   of Histotechnology May 18-21).
>   Please let me know what you find is the best fixative for frozen 
> section morphology.
>
>   Stephen
>
>
>
>
>
>Stephen Peters M.D.
>Vice Chairman of Pathology
>Hackensack University Medical Center
>201 996 4836
>
>Pathology Innovations, LLC
>410 Old Mill Lane,
>Wyckoff, NJ 07481
>201 847 7600
>www.pathologyinnovations.com
>
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)