[Histonet] liver damage detection

[Rene J Buesa]

Caroline:
  Just some points:
  1- first you need to be very familiar and knowledgeable of mice liver normal histology;
  2- I do not understand why you do think that H&E will be sufficient vwithout any previous idea of the type of damage you could expect to find. It is very likely that H&E will not be sufficient after all;
  3- A 30 µm section will be probably useless to detect perhaps subtle chages; you have to do those "special arrangements" and get thinner sections.
  4- Finally, where is this virus supposed to "attack"? Cytoplasm, nuclei? Depending on where it is supposed to act is where you should try to look for changes and it will indicate the type of stain you will need.
  Just some thought that I hope will help you!
  René J.

Caroline Bass <cbass <@t> bidmc.harvard.edu> wrote:
  Dear Histonetters,

I am working on an experimental model of viral liver damage. Does 
anyone have suggestions as to how I should best proceed in 
establishing the type or extent of damage caused? I don't expect 
damage to the extent that the mice die or even show any overt signs 
of illness. There is no noticeable gross change in the liver.

Ideally I would like to establish a biochemical marker of damage such 
as serum ALT as well as a morphological analysis. I figure an H&E 
stain would be enough. I would like to use NBF fixed tissue. Are 
there any suggestions as to the thickness of the liver sections? I 
can reliably section 30 um, anything thinner would require special 
arrangements.

Any suggestions for a kit to measure ALT? A colleague suggested that 
I submit the samples to the hospital's diagnostic lab, but would they 
be able to detect mouse ALT?

Any suggestions would be appreciated. I have never worked with liver 
damage, so I really don't know what to do here.

Thanks in advance,

Caroline



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