[Histonet] validation for CAP
Featherstone, Annette
AFeatherstone <@t> KaleidaHealth.Org
Wed Apr 5 06:05:58 CDT 2006
We have just purchased 2 new Dako immuno stainers and I was wondering what CAP requirements are for validation. The company says that both instruments are validated against each other and therefore you can validate the antibodies on either stainer. I hope this is fact.
Annette Featherstone HT/MLT MT(HEW)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
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Sent: Wednesday, April 05, 2006 05:34
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 29, Issue 6
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Today's Topics:
1. PTH (Richard Cartun)
2. Humedity chamber (cynthia haynes)
3. Fatty breast processing (Orr, Rebecca)
4. neutrophils (Webb, Dorothy L)
5. RE: neutrophils (Monfils, Paul)
6. Sirius red with low contrast between cells cytoplasm and
stained fibers (Guillermo Palao)
7. Re: Humedity chamber (Joanne Mauger)
8. RE: Thanks for the Input (Dave Low)
9. RE: Microtome knife sharpening service/ Microwave accessories
(Justin Thomas)
10. Humidity chamber (Donna Harclerode)
11. Histology Managers, Supervisors, and Bench Techs needed-
Intervirewing and Hiring NOW (Eric Dye (ext 223))
12. Hematoxylin staining of frozen samples, where are the nuclei?
(Guillermo Palao)
13. Qualifications for Histology Supervisor (Lester Raff)
14. Re: humidity chambers (Lori Richey)
15. Re: breast processing (Lori Richey)
16. Teaching Microtomes (Anwar Al-Banaw)
17. Re: Sirius red with low contrast between cells cytoplasm
andstained fibers (John Kiernan)
18. Feedback on Milestone Medical's RHS Series Microwave Tissue
Processor (jenbug812 <@t> aol.com)
19. RE: Sirius red with low contrast between cells cytoplasm
andstained fibers (Tony Henwood)
20. Re: Hematoxylin staining of frozen samples, where are the
nuclei? (Katri Tuomala)
21. Re: neutrophils (Jennifer MacDonald)
22. RE: Thanks for the Input (DDDeltour <@t> mar.med.navy.mil)
23. RE: Thanks for the Input (Kemlo Rogerson)
----------------------------------------------------------------------
Message: 1
Date: Tue, 04 Apr 2006 13:13:52 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] PTH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s432712a.069 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII
Is anyone doing immunohistochemical staining for parathyroid hormone
(PTH) on formalin-fixed, paraffin-embedded tissue? If so, whose
antibody are you using? I just found out that DAKO no longer sells the
rat monoclonal antibody that we had been using.
It would be nice if suppliers notified their customers before they
discontinue a product so that we can find a replacement before we run
out of reagent.
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
------------------------------
Message: 2
Date: Tue, 4 Apr 2006 11:10:55 -0700 (PDT)
From: cynthia haynes <naje1972 <@t> yahoo.com>
Subject: [Histonet] Humedity chamber
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060404181055.97672.qmail <@t> web33006.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello all!
Some one put a website for a humedity chamber for
Immunohistochemistry staining. I think it was
www.scyetex.com. or something like that, would please
put the site address on the histonet again. Thanks in
advance.
Cynthia Haynes H.T.
------------------------------
Message: 3
Date: Tue, 4 Apr 2006 13:23:54 -0500
From: "Orr, Rebecca" <ROrr <@t> enh.org>
Subject: [Histonet] Fatty breast processing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A7FC3A4F98964A44B0B71B7B25EA6F9603AF43C9 <@t> EXCHANGE03.enhnet.org>
Content-Type: text/plain; charset="US-ASCII"
HI Annette,
You have gotten quite a few responses to your question.
I have just one more. When we get huge pieces of fatty breast that is
unfixed, we place the block in the embedder, melt it and let it "soak"
in the paraffin for 2 hours. I have left them in this paraffin bath as
long as 4 hours but am unsure of any damage that could be done,( I
haven't seen any) But 2 seems to be as long as we need. I would think
that this process would cause damage, but since the tissue is unworkable
in the first place, this can only help.
This soaking in paraffin seems to do the trick, whether it's helping
infiltration or simply dehydrating, I imagine it's doing a little bit of
both.
After this process, the blocks are much easier to cut. I don't see any
problem with my IHC stains especially for ER/PR, Her2. These all seem
to stain consistently.
Ultimately, you would want to run your samples as others have
recommended before it gets to the embedding stage.
Hope this helps,
Becky
Becky Orr, CLA,HT(ASCP)
Assistant Manager, Anatomic Pathology
Evanston Northwestern Healthcare
847-570-2771
tm <@t> libero.it>
Message: 5
Date: Tue, 4 Apr 2006 09:26:26 -0400
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
Subject: [Histonet] breast processing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9B4A77DF11463E4FB723D484214AE9BCA55154 <@t> KALEXMB02.KaleidaHealth.org>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have any suggestions for better tissue processing for fatty
tissue such as breast? We are experiencing insufficent dehydration,
clearing and infiltration.
Annette Featherstone HT/MLT
------------------------------
Message: 4
Date: Tue, 04 Apr 2006 13:25:14 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] neutrophils
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<0E394B648E5284478A6CCB78E5AFDA270171FD2A <@t> hpes1.HealthPartners.int>
Content-Type: text/plain; charset="US-ASCII"
Does anyone know of a stain for neutrophils? I believe I had read that
staining with chloroacetate esterase is the way to go, but, I am not
familiar with that stain. Does anyone know of a company that would
have the stain in a kit?? This is for a research project and am rather
stumped! Thanks, as always, fellow Histonetters for your help and
support!!!!
________________________________________
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Message: 5
Date: Tue, 4 Apr 2006 14:37:46 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] neutrophils
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE017176BC <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
I use the Naphthol AS-D Chloroacetate Esterase Kit from Sigma (cat# 91C-1KT)
on frozen sections. It's easy to use and very reliable.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Webb,
> Dorothy L
> Sent: Tuesday, April 4, 2006 11:25 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] neutrophils
>
> Does anyone know of a stain for neutrophils? I believe I had read that
> staining with chloroacetate esterase is the way to go, but, I am not
> familiar with that stain. Does anyone know of a company that would
> have the stain in a kit?? This is for a research project and am rather
> stumped! Thanks, as always, fellow Histonetters for your help and
> support!!!!
> ________________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they are
> addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient, please be
> advised that you have received this e-mail in error and that any use,
> dissemination, forwarding, printing, or copying of this e-mail is strictly
> prohibited.
>
> If you have received this e-mail in error, please immediately notify the
> HealthPartners Support Center by telephone at (952) 967-6600. You will be
> reimbursed for reasonable costs incurred in notifying us.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 6
Date: Tue, 4 Apr 2006 20:58:33 +0200 (CEST)
From: Guillermo Palao <gpbnas <@t> yahoo.es>
Subject: [Histonet] Sirius red with low contrast between cells
cytoplasm and stained fibers
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20060404185833.93523.qmail <@t> web26202.mail.ukl.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello all:
I would really appreciate any suggestions to improve sirius red staining of kidney samples. Although collagen fibers in tissue nicely stain red, cells cytoplasm do not stain yellow, but sort of pinkish, making it very difficult to separate from red-stained extracellular fibers for quantification purposes.
My protocol is as follows:
1. Deparafinize and hydrate samples
2. Stain 1 h in 0.1% Sirius Red in saturated aqueous picric acid
3. Wash 2 min 0.01 N HCl
4. Rinse in water
5. Dehydrate and cover slides
I have tried shorter staining times and rinsing with water before HCl washing without improving results. Any help would be greatly appreciated.
Guillermo
---------------------------------
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------------------------------
Message: 7
Date: Tue, 04 Apr 2006 15:49:36 -0400
From: "Joanne Mauger" <mauger <@t> email.chop.edu>
Subject: Re: [Histonet] Humedity chamber
To: <Histonet <@t> lists.utsouthwestern.edu>,<naje1972 <@t> yahoo.com>
Message-ID: <s432959e.079 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII
Cynthia,
It's www.scytek.com
Jo
>>> cynthia haynes <naje1972 <@t> yahoo.com> 04/04/06 2:10 PM >>>
Hello all!
Some one put a website for a humedity chamber for
Immunohistochemistry staining. I think it was
www.scyetex.com. or something like that, would please
put the site address on the histonet again. Thanks in
advance.
Cynthia Haynes H.T.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 8
Date: Tue, 4 Apr 2006 13:19:41 -0700 (PDT)
From: Dave Low <lowman034 <@t> yahoo.com>
Subject: RE: [Histonet] Thanks for the Input
To: DDDeltour <@t> mar.med.navy.mil, vazquezr <@t> ohsu.edu,
Jackie.O'Connor <@t> abbott.com, Heather.A.Harper <@t> pcola.med.navy.mil
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060404201941.10561.qmail <@t> web32004.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Douglas,
Military personnel DO NOT make rank by working at some
bake sales! Are you insinuating Master Chief Petty
Officers, Sergeant Majors, and Chief Master Sargeants
made rank selling cookies? Rubbish!
I am an Air Force Master Sargeant, worked in histology
for over 17 years out of my 20+ years service. I
worked at the Armed Forces Institute of Pathology in
Washington DC from 1997-2000 and had the pleasure to
work for the Army, Navy, and civilians histology
technicians and managers. The promotions I did see
were from hard work not only from the job but to the
military and civilian communities. It's called the
whole person concept!
In the future please screen your e-mail for
appropriateness before you broadcast to a large
audience like histonet.
Dave Low, MSgt,USAF
HT(ASCP)QIHC
--- DDDeltour <@t> mar.med.navy.mil wrote:
> I work in this environment everyday. I see both
> sides (military and
> civilian) taking shots at each other instead of
> working as a team. I am soon
> leaving the service and you could not pay me enough
> money to supervise or
> work in a military facility. I understand Heather
> and her frustration of
> being left to do the job BUT the military requires
> its members to do things
> outside of the job to get promoted. That is a fact.
> I am living it. I see
> people in my own field get promoted because they did
> some BS bake sale but
> they can't even do a GMS. You can either find a way
> to work it out or find a
> new job. As for the military Pathologist....well
> they are in charge. They
> like that. Everything will change again when the
> next one transfers in. That
> is part of being in a military facility. I have seen
> it for years. The best
> thing to do is go with the flow or just go. I am
> going. Good luck.
>
> Douglas D. Deltour HT(ASCP)
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> On Behalf Of Robyn
> Vazquez
> Sent: Monday, April 03, 2006 12:29 PM
> To: Jackie.O'Connor <@t> abbott.com; Harper, Heather A.,
> CIV
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Thanks for the Input
>
> Sounds like you both work hard...I have been on both
> sides of the
> fence...keep up the good work...she needs all the
> support you can give her
> civilian or military!
> Just my two cents...
>
> Robyn
> OHSU
>
> >>> "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
> 4/3/2006 8:49 AM >>>
> I am thankful and grateful for anyone who ever
> signed up for military
> service - ever - regardless of their job
> description.
>
>
>
>
>
> Heather.A.Harper <@t> pcola.med.navy.mil
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 04/03/2006 10:20 AM
>
>
> To: histonet <@t> lists.utsouthwestern.edu
> cc: (bcc: Jackie M
> O'Connor/LAKE/GPRD/ABBOTT)
> Subject: [Histonet] Thanks for the
> Input
>
>
> I want to thank everybody who responded to my
> message titled..Need Input.
> I
> work for the DOD and I miss simply having a system
> in place. I agree one
> has
> to be flexible, but when you work with military,
> they are entitled to 1 hr
> lunch and 1 hour of PT. My tech works 7-4 and I work
> 6-2. I embed, she
> cuts
> and stains, I gross, accession, order supplies,
> admit bodies in and out of
> the morgue, set up for autopsies, frozens, and when
> my tech has to get
> pulled to do her other command duties, it leaves me
> holding the bag. It
> does get over whelming, and every 2-3 yrs, I get new
> pathologists rotating
> through. I have worked with 7 pathologists in 6 yrs,
> and reservists, and
> this one I just do not know what to think. Just
> keeping an open mind.
> Thanks
> again everybody. Be thankful you are in the civilian
> world.
>
>
>
> Heather
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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------------------------------
Message: 9
Date: Tue, 4 Apr 2006 13:22:18 -0700 (PDT)
From: Justin Thomas <jstn192 <@t> yahoo.com>
Subject: [Histonet] RE: Microtome knife sharpening service/ Microwave
accessories
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20060404202218.93567.qmail <@t> web35701.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Just to let everyone know:
There is finally a company out there that sharpens microtome knifes
(both stainless steel & tungsten) and manufactures plastic microwave
accessories at a low price. I send all our knifes to them and I am
extremely satisfied with the customer service I receive, along with the
pricing. Also, they have made a number of accessories for us and I have
nothing but good words to say about them. The accessory's work well and
with no problem. The best thing about them is the company offers a
warranty with each accessory. If you wish, email me back with your contact
information and I will pass it on to the sharpening and MW accessory
company.
Thanks,
Dr. Thomas
---------------------------------
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------------------------------
Message: 10
Date: Tue, 4 Apr 2006 13:36:57 -0700
From: "Donna Harclerode" <dharclerode <@t> cytoritx.com>
Subject: [Histonet] Humidity chamber
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3DE0F644E093DF4BAE80C254176696A5090706 <@t> mp-mailserver.macropore.com>
Content-Type: text/plain; charset="us-ascii"
Thermo Electron makes a great system with a humidity chamber and a
coverplate that I have used for many years. The slides are held with
the plastic coverplate(the coverplates are suppose to be disposable, but
I rinse them with only DI water and reuse them many times) I use 100ul
of antibody (primary, secondary- etc, normally, but have managed to use
as little as 90ul) to cover almost the whole slide. For buffer wash I
fill up the top of the chamber with a squeeze bottle of PBS and allow to
run through in about 5 minutes. I load the slides add primary, wash,
secondary etc and only take them out for chromagen staining.
The racks worked great when I had the positive control at the top of the
slide and the test below. I do fluorescence secondaries in the
coverplates, but I not do DAB in the coverplates, but flat on the
counter.
For HRP frozens I use the Dako endogenous peroxidase block in the
Sequenza racks. I also use the Dako avidin biotin block in the
coverplates when necessary.
I usually incubate primary overnight in the fridge and the chambers stay
humid for at least 48 hours. It takes a bit of practice to load the
plates, but it is so worth it.
Sequenza Racks - # 73310017 (Holds 10 coverplate assemblies)
Coverplates - 25/pk = # 72110017
50/pk = # 7219950
250/case = # 72110013
http://www.thermo.com/eThermo/CMA/PDFs/Product/productPDF_19578.pdf 4th
page
Donna Harclerode, HT, (ASCP), HTL, QIHC
Scientist / Immunohistochemistry
Cytori Therapeutics
3020 Callan Rd.
San Diego, CA 92121
858-458-0900 ext 5416
dharclerode <@t> cytoritx.com
------------------------------
Message: 11
Date: Tue, 4 Apr 2006 17:13:06 -0400
From: Eric Dye (ext 223) <Eric <@t> ategra.com>
Subject: [Histonet] Histology Managers, Supervisors, and Bench Techs
needed- Intervirewing and Hiring NOW
To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <QlM4M0tLRyYvVCpCIVVgMTU1ODg4MTI5Ng <@t> blkdell3l>
Content-Type: text/plain
Fellow-Histonetters
I'm presently on a search for my best client companies seeking HistoTech Supervisors and Histo Bench Techs. These are mostly fulltime permanent positions. Most of my Histo Techs positions are clinical, although I do have a few Temporary and Research Histo Tech positions as well. Most Histo jobs are dayshift (Except where indicated below)
Here are some of my Hottest Histology Jobs:
1. Ohio (Southwestern Ohio) (Full-time, Perm, Histo Manager)
2. Northern New Jersey (Full-time, Perm and/or Temp, Bench Histo Tech, 1st shift)
3. Rhode Island - Providence (Full-time, Perm, Bench Histo Tech, 2nd Shift)
4. Northern New Jersey (Full-time, Perm, Bench Histo Tech)
5. Ohio (Southwestern Ohio) (Full-time, Perm, Bench Histo Tech)
6. New York (Long Island) (Full-time, Perm, SUPERVISOR Histo Tech)
7. Virginia(South - Coastal) (Full-time, Perm, Bench Histo Tech)
8. Virginia - Metro DC (Full-time, Perm, Lead Histo Tech)
9. South Florida (Full-time, Perm, Supervisor Histo Tech)
10. South Florida (Full-time, Perm, Bench Histo Tech)
11. Mass (Boston) (Part-time, Perm, Bench Histo Tech)
12. West Viriginia (Full-time, Perm, Bench Histo Tech)
The clients are currently interviewing Histology candidates - so if you are interested, please call me today at 1-800-466-9919 ext 223
Thank you,
Eric Dye-Sr Allied Healthcare Recruiter
1-800-466-9919 ext 223
---------------------------------------------------------------
Ategra Systems Inc
Specialists in Permanent & Contract Staffing
800 466 9919 ext 223 - voice
407 671 6075 - fax
eric <@t> ategra.com
To Learn More About Ategra:
http://www.ategra.com
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Message: 12
Date: Tue, 4 Apr 2006 23:22:43 +0200 (CEST)
From: Guillermo Palao <gpbnas <@t> yahoo.es>
Subject: [Histonet] Hematoxylin staining of frozen samples, where are
the nuclei?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20060404212243.32475.qmail <@t> web26209.mail.ukl.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear histonetters,
When I stain samples placed on VWR frosted slides with Gills hematoxylin, nuclei are nicely stained. However, when the same samples are counterstained after passing through all necessary steps involved in immunohistochemistry, hematoxylin staining of nuclei is simply horrible. Is it possible that nuclei are lost in the different washing steps? Please put forward any suggestions you might have.
Guillermo
---------------------------------
LLama Gratis a cualquier PC del Mundo.
Llamadas a fijos y móviles desde 1 céntimo por minuto.
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------------------------------
Message: 13
Date: Tue, 4 Apr 2006 16:23:23 -0500
From: "Lester Raff" <LRaff <@t> lab.uropartners.com>
Subject: [Histonet] Qualifications for Histology Supervisor
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5DA1CA5D0B98A84985B545A24423B822A7AE <@t> UPLAB01.uplab.local>
Content-Type: text/plain; charset="us-ascii"
Hi all,
We are preparing for our first CAP inspection and want to make sure
that my very qualified histology supervisor meets CLIA regs. Does
anyone have a copy of the old federal regs 42CFR493.1427 that defined
minimal qualifications for general supervisor?
Thanks,
Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory
ph: 708-486-0076
fax: 708-486-0080
------------------------------
Message: 14
Date: Tue, 04 Apr 2006 14:41:19 -0700
From: Lori Richey <lrichey <@t> u.washington.edu>
Subject: Re: [Histonet] humidity chambers
To: louise renton <louise.renton <@t> gmail.com>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4432E7FF.4090702 <@t> u.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
We buy Fisher brand absorbant paper, which is plastic coated on one
side. We have it spread flat on the counter and wet with water. We put
the slides flat on the paper, and cover them with a cafeteria tray. This
works well for 100-200 slides a day.
louise renton wrote:
>Hi all
>
>what are folks using as humidity chambers for immuno slides (manual
>method). Are they predominanty made in-house or are there ready made
>commercial products out there?
>
>best regards
>--
>Louise Renton
>Bone Research Unit
>University of the Witwatersrand
>Johannesburg
>South Africa
>"Does a conceited cowboy, only ride on pompous grass?.
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 15
Date: Tue, 04 Apr 2006 14:49:48 -0700
From: Lori Richey <lrichey <@t> u.washington.edu>
Subject: Re: [Histonet] breast processing
To: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4432E9FC.2080100 <@t> u.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Alcoholic Formalin or Penfix in the processor helps some, if the tissue
is grossed in at a reasonable thickness..
Featherstone, Annette wrote:
>Does anyone have any suggestions for better tissue processing for fatty tissue such as breast? We are experiencing insufficent dehydration, clearing and infiltration.
>
>Annette Featherstone HT/MLT
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
>histonet-request <@t> lists.utsouthwestern.edu
>Sent: Sunday, April 02, 2006 13:01
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 29, Issue 2
>
>
>Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
>To subscribe or unsubscribe via the World Wide Web, visit
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>
>
>Today's Topics:
>
> 1. My frustrating experience with double fluorescence staining!
> (Chengming Wang)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Sat, 01 Apr 2006 23:25:00 -0600
>From: "Chengming Wang" <wangche <@t> auburn.edu>
>Subject: [Histonet] My frustrating experience with double fluorescence
> staining!
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <442F0BCC020000AF0001AB19 <@t> groupwise1.duc.auburn.edu>
>Content-Type: text/plain; charset=US-ASCII
>
>Dear All,
>
>I am new with histology, and posted yesterday a message of: Help
>needed!!! Immunofluorescence double staining with mouse lung. Thanks a
>lot for the several helpful responses. As asked, I am here putting on
>my concise protocols for your check:
>
>Sampling: Cut a small cut along the mouse trachea, injected neg 50
>(similar to OCT). Then quick freeze with liquid nitrogen, and the stored
>@ -80; After section with 6 um, slides were fixed with icy acetone for
>5 minutes. Quick dry, and then stored @ -20 till use.
>
>Staining: 1. Washing 3 X 5 mins; Blocking Buffer (10% Rabbit serum; 5%
>BSA; 0.1% Tween 20) for 1 hour at room temperature;
> 2. Tapping away the blocking buffer, and applied first
>antibody for 1 hour; then wash 3 x 5 min'
> 3. 2nd antibody 1 hour; washing 3 x 5min;
> 4. Mounting media, read slides. Count stained with
>DAPI-mounting media.
> 5. For double staining, I did the same thing, except
>using mixed first antibodies and mixed second conjugated antibodies.
>
>Designing: Stain 1: Rabbit anti-mouse antibody, and donkey anti-rabbit
>conjugated with Alexa Fluor 488;
> Stain 2: Rat anti-mouse antibody, and donkey anti-rat
>conjugated Alexa fluor 594;
>
>My problems: The individual staining signals are good. But there is
>some match color between two channels. For example, I could see weak and
>similar green signal when I applied Alexa 594. Basically, I could see
>the weak but true alveoli structure of mouse
> lung.
> Then I performed double staining, I could see
>lots of signal match between red and green channels, which should not be
>true. In this way, I could see basic alveoli structure of mouse
> lung in both green and red channels. By the way,
>I tried to red the slides before staining and after blocking, the
>autofluorescence is very very weak.
>
>I am really frustrating with this situations, and please feel free to
>tell me how I could figure out this problem. Any of your suggestions and
>comments are very much appreciated.
>
>Thanks,
>
>
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Chengming Wang
>DVM, M.S., Ph.D.
>Department of Pathobiology
>College of Veterinary Medicine
>Auburn University
>264 Greene Hall
>Auburn, AL 36849-5519
>
>Voice: (334) 844-2624
>Email: wangche <@t> auburn.edu
>
>
>
>
>------------------------------
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>End of Histonet Digest, Vol 29, Issue 2
>***************************************
>
>
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>
------------------------------
Message: 16
Date: Wed, 5 Apr 2006 00:50:47 +0300
From: "Anwar Al-Banaw" <banaw <@t> HSC.EDU.KW>
Subject: [Histonet] Teaching Microtomes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <59A3C2F593DF494294C4E0E8FFDC9028143D9F <@t> MAIL.HSC.EDU.KW>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
I am planning to get few new manual rotary microtomes for teaching purposes. I heard about "accu-cut" from Tissue-Tek Sakura, have a simple microtome but I want the review of ppl used this machine. The other choices that I have are from Leica and Shandon.
Regards
A AlBanaw
Medical Lab Sci.
Kuwait University
Kuwait
------------------------------
Message: 17
Date: Tue, 04 Apr 2006 17:58:33 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Sirius red with low contrast between cells
cytoplasm andstained fibers
To: Guillermo Palao <gpbnas <@t> yahoo.es>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4432EC09.3010504 <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1; format=flowed
Instead of your steps 3 and 4, rinse in slightly
acidified water. (Your 0.01% HCl should be OK; I
use 0.2-0.5% acetic; about 30 s with agitation).
You can do 2 rinses if there's too much colour in
the water. This step does not extract any bound
dye. Shake off as much acidified water as you can
before the next step.
For your step 5 go directly into the first of 3
changes of 100% alcohol. Agitate slides for about
30 sec in each, then clear (xylene X2) and apply a
coverslip. You lose some of the picric acid in the
dehydration, but there's enough left in the
section to give a yellow colour to everything
except collagen. Nuclei are also yellow, unless
you stain them black before the picri-sirius red.
The long stay in the staining solution extracts
even Weigert's iron-haematoxylin, so a prior
nuclear stain needs to be decidedly overdone.
Junqueira at al (1979, Histochem. J. 11:447-455)
thoroughly studied this method; the paper is well
worth reading.
John Kiernan
Anatomy, UWO
London, Canada
-------------------
Guillermo Palao wrote:
>
> Hello all:
>
> I would really appreciate any suggestions to improve sirius red staining of kidney samples. Although collagen fibers in tissue nicely stain red, cells cytoplasm do not stain yellow, but sort of pinkish, making it very difficult to separate from red-stained extracellular fibers for quantification purposes.
>
> My protocol is as follows:
>
> 1. Deparafinize and hydrate samples
> 2. Stain 1 h in 0.1% Sirius Red in saturated aqueous picric acid
> 3. Wash 2 min 0.01 N HCl
> 4. Rinse in water
> 5. Dehydrate and cover slides
>
> I have tried shorter staining times and rinsing with water before HCl washing without improving results. Any help would be greatly appreciated.
>
> Guillermo
>
>
> ---------------------------------
>
> LLama Gratis a cualquier PC del Mundo.
> Llamadas a fijos y móviles desde 1 céntimo por minuto.
> http://es.voice.yahoo.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Tue, 04 Apr 2006 18:14:10 -0400
From: jenbug812 <@t> aol.com
Subject: [Histonet] Feedback on Milestone Medical's RHS Series
Microwave Tissue Processor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C8264C3B5C9264-1088-88C3 <@t> mblk-d50.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Just wondering if anyone is currently using this tissue processor and if so, can you provide any feedback, good or bad? My company is conducting demos with this product and I would like to be prepared for the sales rep.
Thanks
------------------------------
Message: 19
Date: Wed, 5 Apr 2006 08:27:17 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Sirius red with low contrast between cells
cytoplasm andstained fibers
To: "Guillermo Palao" <gpbnas <@t> yahoo.es>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555237CFDC <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="iso-8859-1"
Drop step 3 and 4 (picric acid is soluble in water)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Guillermo Palao
Sent: Wednesday, 5 April 2006 4:59 AM
To: Histonet
Subject: [Histonet] Sirius red with low contrast between cells cytoplasm andstained fibers
Hello all:
I would really appreciate any suggestions to improve sirius red staining of kidney samples. Although collagen fibers in tissue nicely stain red, cells cytoplasm do not stain yellow, but sort of pinkish, making it very difficult to separate from red-stained extracellular fibers for quantification purposes.
My protocol is as follows:
1. Deparafinize and hydrate samples
2. Stain 1 h in 0.1% Sirius Red in saturated aqueous picric acid
3. Wash 2 min 0.01 N HCl
4. Rinse in water
5. Dehydrate and cover slides
I have tried shorter staining times and rinsing with water before HCl washing without improving results. Any help would be greatly appreciated.
Guillermo
---------------------------------
LLama Gratis a cualquier PC del Mundo.
Llamadas a fijos y móviles desde 1 céntimo por minuto. http://es.voice.yahoo.com _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 20
Date: Tue, 4 Apr 2006 19:05:39 -0400
From: "Katri Tuomala" <katri <@t> cogeco.ca>
Subject: Re: [Histonet] Hematoxylin staining of frozen samples, where
are the nuclei?
To: "Guillermo Palao" <gpbnas <@t> yahoo.es>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004b01c6583c$4a92e220$6a9a9618 <@t> Katri>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Guillermo,
Tissues go through a long procedure with immuno and the most detrimental for
all tissue components is the heat retrieval and to a lesser degree enzyme
digestion. The less well fixed and processed tissue suffers most: connective
tissue is distorted and nuclei stain very poorly.
Hope this explains your problem,
Katri
Katri Tuomala
Hamilton, Ontario, Canada
----- Original Message -----
From: "Guillermo Palao" <gpbnas <@t> yahoo.es>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 04, 2006 5:22 PM
Subject: [Histonet] Hematoxylin staining of frozen samples,where are the
nuclei?
> Dear histonetters,
>
> When I stain samples placed on VWR frosted slides with Gills hematoxylin,
> nuclei are nicely stained. However, when the same samples are
> counterstained after passing through all necessary steps involved in
> immunohistochemistry, hematoxylin staining of nuclei is simply horrible.
> Is it possible that nuclei are lost in the different washing steps? Please
> put forward any suggestions you might have.
>
> Guillermo
>
>
> ---------------------------------
>
> LLama Gratis a cualquier PC del Mundo.
> Llamadas a fijos y móviles desde 1 céntimo por minuto.
> http://es.voice.yahoo.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Tue, 4 Apr 2006 22:31:56 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] neutrophils
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF4D875F29.4A758509-ON88257147.001E63B7-88257147.001E63BF <@t> mtsac.edu>
Content-Type: text/plain; charset="ISO-8859-1"
Napththol AS-D Chloroacetate Esterase is used to identify granulo cytes. This procedure can be done on FFPE tissue,or blood
smears.&nbs usually done on
< -----histonet-bounces
To: Histonet <@t> lists.utsouthwestern.edu
Fr Sent by: Date: 04/04/2006 11:25AM
S Does anyone know of a stain for neutro read that
staining with chloroacetate ester not
familiar with that stain. Does would
have the stain in a kit?? &nb and am rather
stumped! Thanks, a and
support!!!!
___ ______________________ 5F__ This e-mail and any files transmitt and are intended solely for the use of the indi to whom they are addressed. If you are not the intended re or the individual responsible for delivering the e-mail to the
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References
1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
------------------------------
Message: 22
Date: Wed, 5 Apr 2006 05:15:57 -0400
From: DDDeltour <@t> mar.med.navy.mil
Subject: RE: [Histonet] Thanks for the Input
To: lowman034 <@t> yahoo.com, vazquezr <@t> ohsu.edu,
Jackie.O'Connor <@t> abbott.com, Heather.A.Harper <@t> pcola.med.navy.mil
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<3F500F8B416C554EBB21FF16642F72E959CE25 <@t> marxchg03.mar.med.navy.mil>
Content-Type: text/plain
It seems lately some people take bits and pieces of posts and twist them
around for argument sake. I am sure you are not quoting me??
I can speak on the part of the Navy only. Maybe I should have stated that on
my last post. As for the Navy ...While the others are out selling cookies
others must stay behind and pick up the slack. These cookie sellers place
the bake sale on the evaluation which earns them the bullet for a higher
mark. The higher mark means a better chance for promotion. A more
experienced and certified tech may not have the time to sell these cookies
for whatever reason or because they care more about the REAL job than
playing Martha Stewart. Green food coloring is not a counterstain.
-----Original Message-----
From: Dave Low [mailto:lowman034 <@t> yahoo.com]
Sent: Tuesday, April 04, 2006 4:20 PM
To: Deltour, Douglas D. (HM2); vazquezr <@t> ohsu.edu;
Jackie.O'Connor <@t> abbott.com; Harper, Heather A., CIV
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Thanks for the Input
Douglas,
Military personnel DO NOT make rank by working at some
bake sales! Are you insinuating Master Chief Petty
Officers, Sergeant Majors, and Chief Master Sargeants
made rank selling cookies? Rubbish!
I am an Air Force Master Sargeant, worked in histology
for over 17 years out of my 20+ years service. I
worked at the Armed Forces Institute of Pathology in
Washington DC from 1997-2000 and had the pleasure to
work for the Army, Navy, and civilians histology
technicians and managers. The promotions I did see
were from hard work not only from the job but to the
military and civilian communities. It's called the
whole person concept!
In the future please screen your e-mail for
appropriateness before you broadcast to a large
audience like histonet.
Dave Low, MSgt,USAF
HT(ASCP)QIHC
--- DDDeltour <@t> mar.med.navy.mil wrote:
> I work in this environment everyday. I see both
> sides (military and
> civilian) taking shots at each other instead of
> working as a team. I am soon
> leaving the service and you could not pay me enough
> money to supervise or
> work in a military facility. I understand Heather
> and her frustration of
> being left to do the job BUT the military requires
> its members to do things
> outside of the job to get promoted. That is a fact.
> I am living it. I see
> people in my own field get promoted because they did
> some BS bake sale but
> they can't even do a GMS. You can either find a way
> to work it out or find a
> new job. As for the military Pathologist....well
> they are in charge. They
> like that. Everything will change again when the
> next one transfers in. That
> is part of being in a military facility. I have seen
> it for years. The best
> thing to do is go with the flow or just go. I am
> going. Good luck.
>
> Douglas D. Deltour HT(ASCP)
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> On Behalf Of Robyn
> Vazquez
> Sent: Monday, April 03, 2006 12:29 PM
> To: Jackie.O'Connor <@t> abbott.com; Harper, Heather A.,
> CIV
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Thanks for the Input
>
> Sounds like you both work hard...I have been on both
> sides of the
> fence...keep up the good work...she needs all the
> support you can give her
> civilian or military!
> Just my two cents...
>
> Robyn
> OHSU
>
> >>> "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
> 4/3/2006 8:49 AM >>>
> I am thankful and grateful for anyone who ever
> signed up for military
> service - ever - regardless of their job
> description.
>
>
>
>
>
> Heather.A.Harper <@t> pcola.med.navy.mil
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 04/03/2006 10:20 AM
>
>
> To: histonet <@t> lists.utsouthwestern.edu
> cc: (bcc: Jackie M
> O'Connor/LAKE/GPRD/ABBOTT)
> Subject: [Histonet] Thanks for the
> Input
>
>
> I want to thank everybody who responded to my
> message titled..Need Input.
> I
> work for the DOD and I miss simply having a system
> in place. I agree one
> has
> to be flexible, but when you work with military,
> they are entitled to 1 hr
> lunch and 1 hour of PT. My tech works 7-4 and I work
> 6-2. I embed, she
> cuts
> and stains, I gross, accession, order supplies,
> admit bodies in and out of
> the morgue, set up for autopsies, frozens, and when
> my tech has to get
> pulled to do her other command duties, it leaves me
> holding the bag. It
> does get over whelming, and every 2-3 yrs, I get new
> pathologists rotating
> through. I have worked with 7 pathologists in 6 yrs,
> and reservists, and
> this one I just do not know what to think. Just
> keeping an open mind.
> Thanks
> again everybody. Be thankful you are in the civilian
> world.
>
>
>
> Heather
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Message: 23
Date: Wed, 5 Apr 2006 10:33:20 +0100
From: Kemlo Rogerson <kemlo.rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] Thanks for the Input
To: "'DDDeltour <@t> mar.med.navy.mil'" <DDDeltour <@t> mar.med.navy.mil>,
lowman034 <@t> yahoo.com, vazquezr <@t> ohsu.edu, Jackie.O'Connor <@t> abbott.com,
Heather.A.Harper <@t> pcola.med.navy.mil
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<B4FA3DD12D42DA11A5DA00508BAF8649025DFEC8 <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain
Geez I'm losing this!!!! Does the army make cakes? I thought they shot
rifles and things.
You get promoted if you can make a good cake but not if you shoot straight?
Lost it totally.
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
"Following the line of least resistance makes both men and rivers crooked"
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on its
contents: to do so is strictly prohibited and may be unlawful. Please inform
me that this message has gone astray before deleting it. Thank you for your
co-operation
-----Original Message-----
From: DDDeltour <@t> mar.med.navy.mil [mailto:DDDeltour <@t> mar.med.navy.mil]
Sent: Wednesday, April 05, 2006 10:16 AM
To: lowman034 <@t> yahoo.com; vazquezr <@t> ohsu.edu; Jackie.O'Connor <@t> abbott.com;
Heather.A.Harper <@t> pcola.med.navy.mil
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Thanks for the Input
It seems lately some people take bits and pieces of posts and twist them
around for argument sake. I am sure you are not quoting me??
I can speak on the part of the Navy only. Maybe I should have stated that on
my last post. As for the Navy ...While the others are out selling cookies
others must stay behind and pick up the slack. These cookie sellers place
the bake sale on the evaluation which earns them the bullet for a higher
mark. The higher mark means a better chance for promotion. A more
experienced and certified tech may not have the time to sell these cookies
for whatever reason or because they care more about the REAL job than
playing Martha Stewart. Green food coloring is not a counterstain.
-----Original Message-----
From: Dave Low [mailto:lowman034 <@t> yahoo.com]
Sent: Tuesday, April 04, 2006 4:20 PM
To: Deltour, Douglas D. (HM2); vazquezr <@t> ohsu.edu;
Jackie.O'Connor <@t> abbott.com; Harper, Heather A., CIV
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Thanks for the Input
Douglas,
Military personnel DO NOT make rank by working at some
bake sales! Are you insinuating Master Chief Petty
Officers, Sergeant Majors, and Chief Master Sargeants
made rank selling cookies? Rubbish!
I am an Air Force Master Sargeant, worked in histology
for over 17 years out of my 20+ years service. I
worked at the Armed Forces Institute of Pathology in
Washington DC from 1997-2000 and had the pleasure to
work for the Army, Navy, and civilians histology
technicians and managers. The promotions I did see
were from hard work not only from the job but to the
military and civilian communities. It's called the
whole person concept!
In the future please screen your e-mail for
appropriateness before you broadcast to a large
audience like histonet.
Dave Low, MSgt,USAF
HT(ASCP)QIHC
--- DDDeltour <@t> mar.med.navy.mil wrote:
> I work in this environment everyday. I see both
> sides (military and
> civilian) taking shots at each other instead of
> working as a team. I am soon
> leaving the service and you could not pay me enough
> money to supervise or
> work in a military facility. I understand Heather
> and her frustration of
> being left to do the job BUT the military requires
> its members to do things
> outside of the job to get promoted. That is a fact.
> I am living it. I see
> people in my own field get promoted because they did
> some BS bake sale but
> they can't even do a GMS. You can either find a way
> to work it out or find a
> new job. As for the military Pathologist....well
> they are in charge. They
> like that. Everything will change again when the
> next one transfers in. That
> is part of being in a military facility. I have seen
> it for years. The best
> thing to do is go with the flow or just go. I am
> going. Good luck.
>
> Douglas D. Deltour HT(ASCP)
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> On Behalf Of Robyn
> Vazquez
> Sent: Monday, April 03, 2006 12:29 PM
> To: Jackie.O'Connor <@t> abbott.com; Harper, Heather A.,
> CIV
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Thanks for the Input
>
> Sounds like you both work hard...I have been on both
> sides of the
> fence...keep up the good work...she needs all the
> support you can give her
> civilian or military!
> Just my two cents...
>
> Robyn
> OHSU
>
> >>> "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
> 4/3/2006 8:49 AM >>>
> I am thankful and grateful for anyone who ever
> signed up for military
> service - ever - regardless of their job
> description.
>
>
>
>
>
> Heather.A.Harper <@t> pcola.med.navy.mil
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 04/03/2006 10:20 AM
>
>
> To: histonet <@t> lists.utsouthwestern.edu
> cc: (bcc: Jackie M
> O'Connor/LAKE/GPRD/ABBOTT)
> Subject: [Histonet] Thanks for the
> Input
>
>
> I want to thank everybody who responded to my
> message titled..Need Input.
> I
> work for the DOD and I miss simply having a system
> in place. I agree one
> has
> to be flexible, but when you work with military,
> they are entitled to 1 hr
> lunch and 1 hour of PT. My tech works 7-4 and I work
> 6-2. I embed, she
> cuts
> and stains, I gross, accession, order supplies,
> admit bodies in and out of
> the morgue, set up for autopsies, frozens, and when
> my tech has to get
> pulled to do her other command duties, it leaves me
> holding the bag. It
> does get over whelming, and every 2-3 yrs, I get new
> pathologists rotating
> through. I have worked with 7 pathologists in 6 yrs,
> and reservists, and
> this one I just do not know what to think. Just
> keeping an open mind.
> Thanks
> again everybody. Be thankful you are in the civilian
> world.
>
>
>
> Heather
>
>
>
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End of Histonet Digest, Vol 29, Issue 6
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