[Histonet] Helicobacter pylori

Mon Apr 3 14:18:01 CDT 2006

In my experience, I am not aware of any H&E positive cases that were
negative by IHC.  Are your pathologists absolutely certain that these
are bugs?  Did you repeat these cases?  How many sections were
evaluated?  Could you still see the organisms on your negative control? 
The problem with the DAKO antibody is specificity, not sensitivity (it
labels non-H. pylori organisms).

I guess you could send sections from these cases to another laboratory
and see what results they get.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 04/03/06 01:44PM >>>
Has anyone who's using the immuno for helicobacter encountered known
positive cases by H&E to stain negative for immuno? I'm just wondering
how sensitive the antibody is. The cases I've tested with many helico
stain up nicely, but about 10 cases that were diagnosed as
rare/occasional/scattered helicobacter are negative. I'm using the
Dako
antibody and have done testing with enzyme digestion as well as
antigen
retrieval, LSAB+ and EnVision+. Any suggestions would be great.

Thanks,

Jean Taylor, HT(ASCP)QIHC
Immunochemistry
GML - Madison, WI

-----Original Message-----
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Sent: Monday, April 03, 2006 12:09 PM
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Subject: Histonet Digest, Vol 29, Issue 3

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Today's Topics:

   1. humidity chambers (louise renton)
   2. Networking for Field Support Specialist - Histopathology
      TEXAS & Tennessee Regio (John Rynak)
   3. Re: humidity chambers (Phil McArdle)
   4. Thanks for the Input (Heather.A.Harper <@t> pcola.med.navy.mil)
   5. Re: humidity chambers (Joanne Mauger)
   6. Re: Thanks for the Input (Jackie M O'Connor)
   7. Re: Thanks for the Input (Robyn Vazquez)

----------------------------------------------------------------------

Message: 1
Date: Mon, 3 Apr 2006 13:17:00 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: [Histonet] humidity chambers
To: Histonet <@t> lists.utsouthwestern.edu 
Message-ID:
	<e483362e0604030417l1b7b9ad7ycad6e918ee80b8b9 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi all

what are folks using as humidity chambers for immuno slides (manual
method). Are they predominanty made in-house or are there ready made
commercial products out there?

best regards
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"Does a conceited cowboy, only ride on pompous grass?.

------------------------------

Message: 2
Date: Mon, 03 Apr 2006 07:32:26 -0400
From: "John Rynak" <jtrynak <@t> hotmail.com>
Subject: [Histonet] Networking for Field Support Specialist -
	Histopathology	TEXAS & Tennessee Regio
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <BAY101-F403E257C9ADD00089AA6F9BED50 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"

   SciGenium's  Client  is  experiencing  tremendous  growth within
their
   North  American operations. This growth is due to the recent launch
of
   their FDA approved automated histology instrumentation system, and
the
   growing antibody and reagent consumable product line for the
histology
   market.  Due  to  the  new launch of these products they are
currently
   looking

   for   several  Field  Support  Specialist  -  Histopathology,  in
the
   following regions :  Southwest Region (TX Houston or Dallas ) and
TN

   This  position  provides  technical  applications support for our
next
   generation range of automated histopathology products.
   This is a very hands on role where your key tasks will include:

   *   Building an in-depth understanding of our new product
technologies
   and their applications in a diagnostic histopathology environment;

   *   Customer  liaison by providing scientific and laboratory
expertise
   for in-field installations, training and trouble-shooting;

   *  Customer support Help-line for remote problem solving;

   *  Designing and performing experiments to investigate and solve
tough
   technical applications problems; and

   *   Preparing  problem  resolution reports and imparting your
findings
   and  knowledge  to  internal  and  field  based  Product Engineers
and
   Technical Managers.
   To be successful in this role you will possess:

   *  Excellent problem solving and analytical skills

   *   The  ability to communicate with front-end users, technical
design
   engineers and pragmatic sales force members.

   *   A tertiary qualification in a relevant science stream (eg.
Medical
   Laboratory Science)

   *   Practical  experience  in  bench-level  histopathology,
including
   clinical immunohistochemistry

   *  Demonstrated use of automated scientific instrumentation
    BS/ MS in Life Sciences and ASCP Certification
   Interest  in  using  PC  based  software  and maintenance of
automated
   scientific instrumentation will be highly advantageous.
   In addition, you are extremely detailed and analytically minded
with
a
   strong sense of customer focus.

   Interested parties should forward a resume to resume <@t> scigenium.com 

   Send to :

      SciGenium

      PO 380916

      Cambridge, MA

      02238

------------------------------

Message: 3
Date: Mon, 03 Apr 2006 11:16:53 -0400
From: Phil McArdle <pmcardle <@t> ebsciences.com>
Subject: Re: [Histonet] humidity chambers
To: louise renton <louise.renton <@t> gmail.com>,	"Histonet (E-mail)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <44313C65.4060104 <@t> ebsciences.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hello Louise:

I'm a vendor; check out

http://www.ebsstore.com/control/crosssell/~category_id=C/~product_id=H28

01

Best regards,

Phil McArdle

louise renton wrote:
> Hi all
>
> what are folks using as humidity chambers for immuno slides (manual
> method). Are they predominanty made in-house or are there ready made
> commercial products out there?
>
> best regards
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> "Does a conceited cowboy, only ride on pompous grass?.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>   

-- 
Phil McArdle
Microwave Product Manager
Energy Beam Sciences, Inc.
Tel:  800.992.9037 x 341
Cell: 860.597.6796
Fax: 860.653.0422
PMcardle <@t> ebsciences.com 
www.ebsciences.com 
"ADDING BRILLIANCE TO YOUR VISION"

"I hate quotations. Tell me what you know." Ralph Waldo Emerson

------------------------------

Message: 4
Date: Mon, 3 Apr 2006 10:20:15 -0500 
From: Heather.A.Harper <@t> pcola.med.navy.mil 
Subject: [Histonet] Thanks for the Input
To: histonet <@t> lists.utsouthwestern.edu 
Message-ID:

<A7539989B365284E95886432BF68FDDC08CAF1B0 <@t> nhpens-exch2.pcola.med.navy.mi

l>

Content-Type: text/plain

I want to thank everybody who responded to my message titled..Need
Input. I
work for the DOD and I miss simply having a system in place. I agree
one
has
to be flexible, but when you work with military, they are entitled to
1
hr
lunch and 1 hour of PT. My tech works 7-4 and I work 6-2. I embed, she
cuts
and stains, I gross, accession, order supplies, admit bodies in and
out
of
the morgue, set up for autopsies, frozens, and when my tech has to get
pulled to do her other command duties, it leaves me holding the bag. 
It
does get over whelming, and every 2-3 yrs, I get new pathologists
rotating
through. I have worked with 7 pathologists in 6 yrs, and reservists,
and
this one I just do not know what to think. Just keeping an open mind.
Thanks
again everybody. Be thankful you are in the civilian world.

Heather

------------------------------

Message: 5
Date: Mon, 03 Apr 2006 11:42:36 -0400
From: "Joanne Mauger" <mauger <@t> email.chop.edu>
Subject: Re: [Histonet] humidity chambers
To: <pmcardle <@t> ebsciences.com>,<louise.renton <@t> gmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s4310a3c.007 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII

Louise,

If you are staining only a few slides(about 10),a plastic slide
box(100
count) works well. Place slides flat across the top. You can close the
lid, and keep them moist!

Jo

>>> Phil McArdle <pmcardle <@t> ebsciences.com> 04/03/06 11:16 AM >>>
Hello Louise:

I'm a vendor; check out

http://www.ebsstore.com/control/crosssell/~category_id=C/~product_id=H28

01

Best regards,

Phil McArdle

louise renton wrote:
> Hi all
>
> what are folks using as humidity chambers for immuno slides (manual
> method). Are they predominanty made in-house or are there ready made
> commercial products out there?
>
> best regards
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> "Does a conceited cowboy, only ride on pompous grass?.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
>   

-- 
Phil McArdle
Microwave Product Manager
Energy Beam Sciences, Inc.
Tel:  800.992.9037 x 341
Cell: 860.597.6796
Fax: 860.653.0422
PMcardle <@t> ebsciences.com 
www.ebsciences.com 
"ADDING BRILLIANCE TO YOUR VISION"

"I hate quotations. Tell me what you know." Ralph Waldo Emerson

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 6
Date: Mon, 3 Apr 2006 10:49:29 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Thanks for the Input
To: Heather.A.Harper <@t> pcola.med.navy.mil 
Cc: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <OFB862F16F.553B2D7B-ON86257145.0056916F <@t> abbott.com>
Content-Type: text/plain; charset="us-ascii"

I am thankful and grateful for anyone who ever signed up for military 
service - ever - regardless of their job description.

Heather.A.Harper <@t> pcola.med.navy.mil 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
04/03/2006 10:20 AM

        To:     histonet <@t> lists.utsouthwestern.edu 
        cc:     (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT)
        Subject:        [Histonet] Thanks for the Input

I want to thank everybody who responded to my message titled..Need
Input. 
I
work for the DOD and I miss simply having a system in place. I agree
one

has
to be flexible, but when you work with military, they are entitled to
1
hr
lunch and 1 hour of PT. My tech works 7-4 and I work 6-2. I embed, she

cuts
and stains, I gross, accession, order supplies, admit bodies in and
out
of
the morgue, set up for autopsies, frozens, and when my tech has to get
pulled to do her other command duties, it leaves me holding the bag. 
It
does get over whelming, and every 2-3 yrs, I get new pathologists
rotating
through. I have worked with 7 pathologists in 6 yrs, and reservists,
and
this one I just do not know what to think. Just keeping an open mind. 
Thanks
again everybody. Be thankful you are in the civilian world.

Heather

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 7
Date: Mon, 03 Apr 2006 09:29:17 -0700
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: Re: [Histonet] Thanks for the Input
To:
Jackie.O'Connor <@t> abbott.com,	Heather.A.Harper <@t> pcola.med.navy.mil

Cc: histonet <@t> lists.utsouthwestern.edu 
Message-ID: <s430eaf6.060 <@t> OHSU.EDU>
Content-Type: text/plain; charset=us-ascii

Sounds like you both work hard...I have been on both sides of the
fence...keep up the good work...she needs all the support you can give
her civilian or military!
Just my two cents...

Robyn
OHSU

>>> "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com> 4/3/2006 8:49 AM
>>>
I am thankful and grateful for anyone who ever signed up for military 
service - ever - regardless of their job description.

Heather.A.Harper <@t> pcola.med.navy.mil 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
04/03/2006 10:20 AM

        To:     histonet <@t> lists.utsouthwestern.edu 
        cc:     (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT)
        Subject:        [Histonet] Thanks for the Input

I want to thank everybody who responded to my message titled..Need
Input. 
I
work for the DOD and I miss simply having a system in place. I agree
one

has
to be flexible, but when you work with military, they are entitled to
1
hr
lunch and 1 hour of PT. My tech works 7-4 and I work 6-2. I embed, she

cuts
and stains, I gross, accession, order supplies, admit bodies in and
out
of
the morgue, set up for autopsies, frozens, and when my tech has to get
pulled to do her other command duties, it leaves me holding the bag. 
It
does get over whelming, and every 2-3 yrs, I get new pathologists
rotating
through. I have worked with 7 pathologists in 6 yrs, and reservists,
and
this one I just do not know what to think. Just keeping an open mind. 
Thanks
again everybody. Be thankful you are in the civilian world.

Heather

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

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End of Histonet Digest, Vol 29, Issue 3
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