[Histonet] brain tissue

[Charles Scouten]

May I ask, are the brains perfused or immersed?  Perfusion gives better tissue quality, since the whole brain is a big chunck for immersion. Interior poorly fixed is one interpretation of the brittle problem.  

I have not used paraffin, can cannot speak to the inclusion problem.  Why not freeze the brain and cut whole sections?

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hernan Aldana Marcos
Sent: Thursday, September 29, 2005 2:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] brain tissue 

Dear Histonetters
I work with rat´s brains fixed in paraformaldehyde. I have problems with paraffin inclusion and section. I always obtain brittle sections.
Help me
Thanks in advance

Dr. Hernán Aldana Marcos
haldana <@t> unimoron.edu.ar
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