[Histonet] IHC questions

[Steven Coakley]

I'm staining rat liver for anti-Brdu next week, AEC with crystal mount.  I'm assuming the aquesous Hematoxylin mentioned in my "old" procedure referes to Mayers.  Is that correct?
Is 5 minutes sufficient and can scotts be used for bluing or should I use a tap rinse?
 
I know fixation is routinely 24 hours.  To assure complete fixation would 48 hours work ok.  I need to do AR anyways.  Thanks for everyones answers.  Been doing this for over 13 years but only routine.  This IHC, IF, and research stuff is a "tad" new.
 
Thanks again,
 
Steve
 
t"T"t
 

		
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