[Histonet] Mayer's Haemalum reactive

[Massimo]

Dear Histonetters,


I'd like to apologize if my question will sound too basic.

I have stained a tissue with Mayer's Haemalum reactive.

After I have put the specimen in water at a Ph a bit over 7, I would be expected a nuclear colour change from red "bordeaux" to blue.

But it didn't happen.

I fixed the specimen with Bouin's liquid and I clarified with Histoclear.

I wonder why.

Have you seen a behaviour of this kind?



Thanks in advance for your help,

 

Best Regards,

 

Massimo