[Histonet] Superfrost Plus slides

[mucram11 <@t> comcast.net]

Cathy,

You should also be sure you are roatating your slides so the oldest lot numbers are used first.  The coatings are not permenant and can lose surface attraction over time and with exposure to light, heat and air.  The expiration date is from the time they are manufactured, not when they arrive to you.  Be sure your supplier is sending you fresh slides that have not been sitting in a warehouse for months with varying temperatures and conditions.

Hope this helps.

Pam Marcum

-------------- Original message -------------- 

> We are using VWR Superfrost Plus. We do hundreds of Immunos per week and have 
> had no such problems. 
> 
> ________________________________ 
> 
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Cathy 
> Malcontenti-Wilson 
> Sent: Wed 9/28/2005 3:33 AM 
> To: histonet <@t> lists.utsouthwestern.edu 
> Subject: [Histonet] Superfrost Plus slides 
> 
> 
> 
> Dear All, 
> OK so question regarding the superfrost slides. 
> I use them for immunostaining and I remember a few years ago when cutting 
> sections and orientating them in the waterbath, if I got it wrong well bad 
> luck because the sections were well and truly stuck within a few seconds. 
> But now I can orientate them no problem, they lift off easily. 
> Even though they look like most of the sections are still attached, I am 
> getting all this strange background immunostaining around the edges of the 
> tissues and sometimes it looks as though something has been damaging the 
> tissue around the edges as well. I remember a while ago there was some 
> discussion on superfrost slides on histonet but I cant remember what the 
> conclusion was. 
> Has anyone noticed any changes with the superfrost slides, or do they 
> recommend one brand over another? 
> We use Menzel brand and have tried two suppliers, and both the superfrost 
> and the superfrost plus slides. 
> Thanks in advance. 
> Cathy 
> 
> 
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