[Histonet] Superfrost Plus slides

Cathy Malcontenti-Wilson cmalc <@t> unimelb.edu.au
Wed Sep 28 03:33:11 CDT 2005


Dear All,
OK so question regarding the superfrost slides.
I use them for immunostaining and I remember a few years ago when cutting 
sections and orientating them in the waterbath, if I got it wrong well bad 
luck because the sections were well and truly stuck within a few seconds. 
But now I can orientate them no problem, they lift off easily.
Even though they look like most of the sections are still attached, I am 
getting all this strange background immunostaining around the edges of the 
tissues and sometimes it looks as though something has been damaging the 
tissue around the edges as well. I remember a while ago there was some 
discussion on superfrost slides on histonet but I cant remember what the 
conclusion was.
Has anyone noticed any changes with the superfrost slides, or do they 
recommend one brand over another?
We use Menzel brand and have tried two suppliers, and both the superfrost 
and the superfrost plus slides.
Thanks in advance.
Cathy




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