[Histonet] IHC background problems on chicken embryos

[Katri Tuomala]

Hi Histonetters,
A colleague of mine is trying to get VWF (Dakocytomation) and VEGF(R&D 
Systems) working on chicken tissue. The antibodies are performing well on 
human and rat tissue, but excessive background is the problem with the 
chicken. Would the blocking serums (normal goat and normal rabbit 
respectively) be the problem as well as the biotinylated secondary reagents 
raised in goat and rabbit? My experience is strictly with human tissues, so 
I am stumped. To my knowledge neither of these antibodies have been proven 
to work with chicken, so maybe that is the problem.
Any advice from "chicken" people?

Katri

Katri Tuomala
Hamilton, Ontario, Canada