[Histonet] Low temp Antigen Retrieval for Bone Sections

[Monfils, Paul]

I haven't tried this method of antigen retrieval, but TEG buffer is
Tris-EGTA buffer. The usual formula (I have seen a couple of variations) is:

Distilled water 1,000 ml
Trisma base  1.21 gram
EGTA  0.19 gram

The usual target pH is between 8.95 and 9.0

EGTA is ethylene glycol bis(B-aminoethyl)-N,N,N',N' tetraacetic acid.

(The capital "B" in the above name represents "beta".  My email program
doesn't support the symbol.)

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Kim
> Merriam
> Sent: 	Monday, September 26, 2005 11:34 AM
> To: 	Histonet
> Subject: 	[Histonet] Low temp Antigen Retrieval for Bone Sections
> 
> Hello All,
>  
> I will be performing some IHC on mouse femurs (about 12 or so different
> antibodies).  In the past, I have had a lot of trouble keeping these
> sections on the slides during HIER.  I did a quick search on the archives
> and there were lots of suggestions, but nothing definitive.
>  
> I was reading an article about "low temp AR", overnight at 60C in TEG
> buffer, pH 9.0. Has anyone tried this method?  Also - what does TEG buffer
> stand for?
>  
> Any help would be greatly appreciated.
>  
> Kim
> 
> 
> Kim Merriam
> Novartis
> Cambridge, MA
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