[Histonet] RNAse decontamination in cryostats

[Gayle Callis]

A friend who is an expert at LCM, dealing with collecting frozen sections 
for RNA work, wipes down the whole inside of the cryostat with 70% 
alcohol.  You could make this up with RNAas away if you wanted to.  She 
wipes down all surfaces, including under the sliding glass door/lid of the 
cryostat.  If you wish I will put you in contact with her - she is not a 
Histonet subscriber, so you can discuss any fine tuning of cryostat RNase 
decontamination.

01:28 PM 9/23/2005, you wrote:
>My question is similar to the current topic of discussion about
>decontaminating cryostats, except that I need to remove any potential RNAse
>activity.  I have been cleaning the disposable blade and the blade holder
>with 70% alcohol, which hasn't been shown to eliminate the pernicious little
>buggers, but the commercial products such as RNAse away (Ambion) etc, are
>water based and would freeze on the blade/blade holder which would make me
>suspicious of their effectiveness.  I am not able to continuously thaw the
>cryostat and then use the products, as I would like to do daily.  I am
>wondering if anyone has any ideas or potential products that would work.
>Thanks
>
>Patrick Laurie, HT (ASCP)
>Neurogenomics Laboratory
>Benaroya Research Institute
>1201 9th Ave
>Seattle, Wa  98101
>(206) 341-0681
>
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)