[Histonet] Antigen retreival on mouse GFAP.

[Jan Shivers]

In my experience with various mammals, antigen retrieval is not needed for
GFAP IHC.  I run mine (using Dako's rabbit anti-cow GFAP) at 30 minutes
primary incubation (3 ug/ml Ab working concentration), 30 minutes
anti-rabbit IgG EnVision+/HRP, and 10 minutes AEC chromogen.

Jan Shivers
U of MN Vet Diag Lab

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----- Original Message ----- 
From: <Susan.Ferrigon <@t> sanofi-aventis.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, September 23, 2005 5:27 AM
Subject: [Histonet] Antigen retreival on mouse GFAP.






Hi

Does anyone have any experience with using trypsin as the method of antigen
retreival when staining GFAP on mouse brains???  Any advice on
concentration, temperature and incubation time would be appreciated.

Thanks


Susan.