[Histonet] Antigen retreival on mouse GFAP.

[Geoff McAuliffe]

Hi Susan:

    I don't know why you would need antigen retrieval for GFAP on mouse 
brain. Most people I know use DAKO's rabbit anti-cow antibody, catalogue 
# Z0334. It works very well on both fixed, frozen sections and on 
formalin-fixed wax embedded tissue. With a Vector ABC Elite kit my 
dilution for GFAP primary is 1:2000 or even more dilute, depending on 
how I intensify the DAB. I also get great results with fluorescence. 
Perhaps your trouble is somewere else?

Geoff

Susan.Ferrigon <@t> sanofi-aventis.com wrote:

>Hi
>
>Does anyone have any experience with using trypsin as the method of antigen
>retreival when staining GFAP on mouse brains???  Any advice on
>concentration, temperature and incubation time would be appreciated.
>
>Thanks
>
>Susan.
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
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