[Histonet] Let's Play solve this problem....

[Timothy Macatee]

Histonetters Assemble!

I'm having a recurring problem with my H & E stained slides.  The staining
is weak, especially away from the edges of the tissue.  It looks almost like
there is still an opaque paraffin haze to the tissue.  It could be a
processing problem because it occurs more towards the middle of the section.
Is something not getting into the tissue far enough?  Is it Fixative (PFA or
Formalin), or processing reagents?  Or am I just whistling dixie?

Tim Macatee
NYU Medical Center
Research Histology Core