[Histonet] (no subject)

[Patrick Laurie]

I am trying to stain for motor neurons on frozen 8um sections for LCM.  I
have been working with the .5% Cresyl Violet Acetate stain (Carson's version
of Vacca's stain) and all of it's derivatives.  I am trying to counter the
differentiation of the alcohols used to completely dehydrate the sections
and leaving very little stain left.  I am aiming for RNA preservation, so
length of time in the stain is important.  I would appreciate any tips.
Thanks,

Patrick Laurie HT (ASCP)
Neurogenomics Laboratory
Benaroya Research Institute
1201 9th Ave
Seattle, Wa  98101
(206) 341-0681