[Histonet] Ventana Benchmark

[Sebree Linda A.]

We always pH our reaction buffer and it tends to be in the 7.2 to 7.4
range.  We don't pH any of the other proprietary buffers, i.e. CC1, CC2,
EZ Prep from Ventana.  I would consult VMS and see what they say about
this subject; we would be interested in knowing as well.  I do remember
that I questioned them once about pHing the EZ Prep and they said not to
because its basically a detergent.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy
Johnson
Sent: Monday, October 31, 2005 1:52 PM
To: Histonet (E-mail)
Subject: [Histonet] Ventana Benchmark


Here is a question to all those out there that have a Ventana Benchmark.
We are going to be inspected by CAP sometime next year.  Our lab has
gotten the CAP checklist and one of the questions has to do with pH of
any of the
bulk fluids on the Ventana.  Our question to you all is:     Do any of
you
routinely pH the EZ Prep, Reaction buffer and the CC1??  I f you do can
you share with us what your ranges are? Thanks 
Amy Johnson


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