[Histonet] Re: Red HRP chromagen/substrate

Johnson, Teri TJJ <@t> Stowers-Institute.org
Thu Oct 27 14:24:14 CDT 2005


It depends on what you consider "best".  As Gayle pointed out, for some
it's endpoint color.  For others, it's permanence. AEC needs to be
mounted in aqueous mountant because it's not stable in organic solvents
(even the polyvinyl alcohol in some of the aqueous mountants can cause
fading!).  The way to get around this is to either use a permanent
chromogen (Vector Nova Red), or you can use Crystal mount to cover the
AEC stained tissue sample and allow to dry.  We always coverslip it with
permanent mountant for better visualization under the microscope.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

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