[Histonet] Decloaking Chamber (high pressure cooker)

Jan Shivers shive003 <@t> umn.edu
Thu Oct 27 13:01:35 CDT 2005

I use a Decloaking Chamber for Chronic Wasting Disease slides, and yes, 20
minutes is the correct setting for that particular test, with a 25 minute
cooldown.  Most other IHC tests don't require a lengthy setting on the

I'd recommend contacting Biocare to do this for you.  They came and
installed the settings on my chambers.

Jan Shivers
U of MN Vet Diag Lab

----- Original Message ----- 
From: "wen eng" <weneng2004 <@t> yahoo.com>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, October 27, 2005 12:27 PM
Subject: [Histonet] Decloaking Chamber (high pressure cooker)

> Hello,
> Does anybody use Decloaking Chamber from Biocare Medical? How should I set
the SP1 temperature and time if I need 20 minutes treatment? The factory
setting is 125 degree 30 seconds and the tech support said 20 minutes is too
long. She suggested me to keep 30 seconds. I still want the suggestion from
here. How do you set your program?
> Thanks!
> Wen
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