[Histonet] Help!! We have a problem with a Mounting Media
gcallis <@t> montana.edu
Thu Oct 27 12:30:02 CDT 2005
Is anyone experiencing this problem with Protocol Mounting Medium T,
toluene solvent based from Fisher.
They take 50 mls and put it into a bottle with a secure, air tight, solvent
resistant lid in order to not dip into stock bottle all the time.
If they use the mounting media absolutely fresh after aliquoting, the
mounting media drys nicely, everything is fine. They are coverslipping
cytospin stained blood cells, Diff Quik stained, and dry without any
immersion oil used before coverslipping.
If they let the Protocol media aliquot, even though in an air tight, new
bottle, sit around, they notice a layer or some type of separation within
top layer of media. When they use this now separated aliquot, stirred,
and then try to dry slide, the coverslip still slips and slides around -
failure of drying!! Very messy!
Something is happening, either evaporation of toluene to change ratio of
solvent to acrylic resin, maybe something else i.e faulty
manufacturing/preparation of this media? This is a total mystery to me as
I have done this type of aliquoting with other mounting medias for years
and NEVER had a problem except to add solvent to restore media - thick to
thinner. Nor I have never experienced layering/separation phenomena with
my other mounting media (Richard Allan brand or Permount). After
coverslipping, the latter mounting media set up and dry nicely with no
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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