[Histonet] Eosin-Hematoxylin solution according to Ehrlich(from Sigma)

[Jackson, Johanna]

Hi,
 
I need to stain frozen rat brain sections (15um) with H&E.  As I'm new to this I bought the ready-made Eosin-Hematoxylin solution according to Ehrlich from Fluka/Sigma.  Does anyone have a protocol for using ready-made H&E solutions?  It seems a bit confusing as I can't do the steps, such as washing etc, that usually go between the haematoxylin and eosin.
 
Many thanks,
 
Jo
 
 
Stem Cell Imaging
MRC Clinical Sciences Centre
Imperial College London
Hammersmith Hospital Campus
Du Cane Road
London
W12 0NN