[Histonet] RE: PCNA versus Ki67
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Wed Oct 26 03:01:41 CDT 2005
As been said before, you will find more positive cells with PCNA than
with Ki67. As far as I know is due to a different half-life of these
antigens. That's the reason why also cells in the G0 phase might be
But, if you're in doubt whether to use PCNA or Ki67, why
not visualizing both of them in double staining? Since you have a
mouse anti-PCNA and rabbit anti-Ki67 (LabVision/Neomarkers) life is
simple here. Prepare a cocktail of these primaries (appropriately
diluted of course). Than, prepare a 1:1 cocktail of
PowerVision-anti-rabbit/AP and PowerVision-anti-mouse/HRP
(ImmunoVision) and incubate for 60 min. Develop AP and HRP activity
respectively in blue (Vector Blue or home-made Fast Blue BB) and red
(AEC or Vector NovaRed). Done!!!
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
Date: Mon, 24 Oct 2005 17:44:40 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] PCNA versus Ki67
To: histonet <@t> lists.utsouthwestern.edu
I have been a big proponent of using Ki67 instead of PCNA as a more
proliferation marker after reading that PCNA may in fact carry over
Recently, some of my colleagues have been clamoring for PCNA.
I'd be interested to hear which marker other labs are using.
Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
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