[Histonet] does fixation kill native EGFP or RFP fluorescence?

Caroline Bass cbass <@t> bidmc.harvard.edu
Tue Oct 25 16:46:46 CDT 2005


My boss insists that EGFP or RFP expressing tissue will lose the  
native fluorescence if fixed.  Is this true?  I know it isn't true  
for tissue culture, but he is very insistent that it will occur.

I have cut the liver into blocks and immersion fixed them in 10%NBF  
overnight at 4 degrees celcius..  If I cut a thin section of fresh  
tissue I can see a lot of signal.  He insists I am wasting my time by  
sectioning the blocks.

Any advice would be appreciated.



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