[Histonet] does fixation kill native EGFP or RFP fluorescence?
cbass <@t> bidmc.harvard.edu
Tue Oct 25 16:46:46 CDT 2005
My boss insists that EGFP or RFP expressing tissue will lose the
native fluorescence if fixed. Is this true? I know it isn't true
for tissue culture, but he is very insistent that it will occur.
I have cut the liver into blocks and immersion fixed them in 10%NBF
overnight at 4 degrees celcius.. If I cut a thin section of fresh
tissue I can see a lot of signal. He insists I am wasting my time by
sectioning the blocks.
Any advice would be appreciated.
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