[Histonet] New product to solve autofluorescence woes

[Gayle Callis]

Dear H'netters

If you are doing immunofluorescence staining on fixed and permeabilized 
cells and tissues and suffer from autofluorescence, there are new things on 
the horizon.  A new product is out although I have not tried it, but am 
curious to know if it works.

Molecular Probes now has Image-IT signal enhancer, cat#136933 which is 
applied before you start your staining.

It would certainly be worth a try for those who use paraformaldehyde or NBF 
fixation, and possibly for FFPE tissue sections destined for 
immunofluorescence staining.  and maybe for PFA fixed frozen sections with 
GFP?

Contact their tech services if you have any questions about this new 
product and you can read about it online at 
www.probes.invitrogen.com    ------   I think it would be worth trying.

If anyone has used it , please post your results - it would be a huge help 
to others performing immunofluorescent staining and suffering from 
autofluorescence woes to know if this works.



Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)