[Histonet] New product to solve autofluorescence woes
gcallis <@t> montana.edu
Tue Oct 25 11:37:23 CDT 2005
If you are doing immunofluorescence staining on fixed and permeabilized
cells and tissues and suffer from autofluorescence, there are new things on
the horizon. A new product is out although I have not tried it, but am
curious to know if it works.
Molecular Probes now has Image-IT signal enhancer, cat#136933 which is
applied before you start your staining.
It would certainly be worth a try for those who use paraformaldehyde or NBF
fixation, and possibly for FFPE tissue sections destined for
immunofluorescence staining. and maybe for PFA fixed frozen sections with
Contact their tech services if you have any questions about this new
product and you can read about it online at
www.probes.invitrogen.com ------ I think it would be worth trying.
If anyone has used it , please post your results - it would be a huge help
to others performing immunofluorescent staining and suffering from
autofluorescence woes to know if this works.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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