[Histonet] GFP in Paraffin Sections?

Favara, Cynthia (NIH/NIAID) cfavara <@t> niaid.nih.gov
Mon Oct 24 17:03:53 CDT 2005

Good advice from Gayle. I have yet to be successful her with GFAP on
frozens and need to come back with an anti -GFP. The most sensitive
antibody I have found is from Roche, cocktail of 2 monoclonal mouse
Ab's. I have not had a problem with mouse on mouse as we are usually
dealing with neuronal tissue. 

Jamie Erickson did a poster around 98 at NSH and will send you her
conclusions. You could look in the archives for her information.


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

The information in this e-mail and any of its attachments is
confidential and may contain sensitive information. It should not be
used by anyone who is not the original intended recipient. If you have
received this e-mail in error please inform the sender and delete it
from your mailbox or any other storage devices. National Institute of
Allergy and Infectious Diseases shall not accept liability for any
statements made that are sender's own and not expressly made on behalf
of the NIAID by one of its representatives

-----Original Message-----
From: Monfils, Paul [mailto:PMonfils <@t> Lifespan.org] 
Sent: Monday, October 24, 2005 10:25 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] GFP in Paraffin Sections?

Does anyone know if GFP (Green Fluorescent Protein) maintains its
fluorescence through paraffin processing?  Can the fluorescence be
viewed in
an otherwise unstained paraffin section?  After deparaffinization,
the section be coverslipped with an aqueous medium, or the standard
used for paraffin sections?

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list