AW: [Histonet] Formaldehyde concentration measurement

[Gudrun Lang]

We use 8% Formaldehyd. 
What would be the right amounts of reagents to test this concentration?
Can you help?

Gudrun Lang



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Tony
Henwood
Gesendet: Freitag, 21. Oktober 2005 00:14
An: Maray Weirauch; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Formaldehyde concentration measurement

The following might be of use:

Test for validity of formalin concentrations


Principle:

The Schiff's reagent (PAS stain) is used to detect aldehydes and In this
technique it is used to titrate a solution of formalin The concentration of
which is unknown.


Solutions:

1.	1 ml of formalin fixative to be tested.
2.	5 ml of 2.4% aqueous sodium bisulphite - prepare fresh.
3.	Schiffs reagent.


Procedure:

1.	Add 1 ml of formalin to 5 ml of sodium bisulphite.
2.	Mix and allow to react for 15 minutes at room temperature stirring
from time to time.
3.	Add 100µl of Schiff reagent.


Results:

If solution turns a deep violet than initial concentration of formalin is in
excess of 4% (i.e. 1.6% formaldehyde).


Reference:

Jaspers, B., J. Histotechnol (1987) 10 (4): 263-265.



Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maray
Weirauch
Sent: Friday, 21 October 2005 5:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde concentration measurement


Hi Histonetters,
Does anyone know of a simple, inexpensive way to measure the formaldehyde
concentration in a prepared, buffered formalin fixative? Thanks!  Maray

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