[Histonet] Formaldehyde concentration measurement

Tony Henwood AnthonyH <@t> chw.edu.au
Thu Oct 20 17:13:42 CDT 2005

The following might be of use:

Test for validity of formalin concentrations


The Schiff's reagent (PAS stain) is used to detect aldehydes and In this technique it is used to titrate a solution of formalin The concentration of which is unknown.


1.	1 ml of formalin fixative to be tested.
2.	5 ml of 2.4% aqueous sodium bisulphite - prepare fresh.
3.	Schiffs reagent.


1.	Add 1 ml of formalin to 5 ml of sodium bisulphite.
2.	Mix and allow to react for 15 minutes at room temperature stirring from time to time.
3.	Add 100µl of Schiff reagent.


If solution turns a deep violet than initial concentration of formalin is in excess of 4% (i.e. 1.6% formaldehyde).


Jaspers, B., J. Histotechnol (1987) 10 (4): 263-265.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maray Weirauch
Sent: Friday, 21 October 2005 5:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde concentration measurement

Hi Histonetters,
Does anyone know of a simple, inexpensive way to measure the formaldehyde concentration in a prepared, buffered formalin fixative? Thanks!  Maray

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