[Histonet] Nissl Staining on Thick Sections

[Monfils, Paul]

Does anyone routinely do Nissl staining on 30 to 40 micron frozen sections?
That's what the current project calls for, and I am finding that Nissl
methods designed for 6 to 8 micron sections don't work well on the thick
sections. They don't differentiate properly.  If anyone has a Nissl protocol
that works well on such thick sections, I would be grateful for any
information.

Paul M.