[Histonet] xylene substitutes

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Oct 17 10:21:47 CDT 2005

If you are looking for xylene substitutes because you are trying to avoid the chemical
hazard that xylene has, all other xylene substitutes are also hazardous in some level
and none work as well as xylene.
If you are trying to avoid the hazard, you have to eliminate aromatic substances 
altogether and go for an aliphatic reagent.
Since attachments are supposed not to be posted in Histonet I am sending a procedure
directly to your e-mail address. This procedure retains fluorescence signals also.
Rene J.

yasushi nakagawa <nakagawa <@t> umn.edu> wrote:
Hi all,
I am wondering how good xylene replacements are compared with xylene. 
We use mouse brain sections (mostly 20um-thick cryosections, and some 
thicker sledge sections) and do immunostaining with cy-2/-3/-5 as 
well as Alexafluor conjugated secondary antibodies, GFP/RFP/CFP 
detection, and DAB reaction/Nissl staining. I wonder if someone knows 
long-term signal (color and fluorescence) retention and tissue 
quality when you use xylene substitutes before DPX mounting. I find 
some in EM Sciences catalog and would like to ask you if you have 
recommendations for particular products.
Thank you.

Yasushi Nakagawa
Department of Neuroscience
Stem Cell Institute
University of Minnesota

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