[Histonet] xylene substitutes

[yasushi nakagawa]

Hi all,
I am wondering how good xylene replacements are compared with xylene.  
We use mouse brain sections (mostly 20um-thick cryosections, and some  
thicker sledge sections) and do immunostaining with cy-2/-3/-5 as  
well as Alexafluor conjugated secondary antibodies, GFP/RFP/CFP  
detection, and DAB reaction/Nissl staining. I wonder if someone knows  
long-term signal (color and fluorescence) retention and tissue  
quality when you use xylene substitutes before DPX mounting. I find  
some in EM Sciences catalog and would like to ask you if you have  
recommendations for particular products.
Thank you.

Yasushi Nakagawa
Department of Neuroscience
Stem Cell Institute
University of Minnesota