[Histonet] (no subject)
Rene J Buesa
rjbuesa <@t> yahoo.com
Fri Oct 14 12:42:00 CDT 2005
I used the DAKO CD117 (rabbit Ig) at 1:25 with HIER at pH 6.0 in the steamer (20 min in + 20 min out) without any problem (in human tissue with human melanoma control).
Are you running a "other than rat" parallel control to be sure that your protocol is working OK?
b003046 <@t> nf.au.dk wrote:
Hi, im trying to get the DAKO polyclonal rabbit anti-human c-kit (CD117) to work
in rats in paraffin. I have tryed using antigen retrieval in microwave and down
to 1:50 dilution. Also I have tryed the C-19 c-kit from Santa Cruz and used
both on paraffin and frozen tissue. It just doesnt want to work! Can anybody
Mette K. Hagensen
Department of Cardiology B, Research Unit
Aarhus University Hospital, Skejby Hospital
8200 Aarhus N
Phone: +45 89496237
Fax: +45 89496009
Mail: b003046 <@t> nf.au.dk
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
Yahoo! Music Unlimited - Access over 1 million songs. Try it free.
More information about the Histonet